6/25/2019

10 Ways to Turn Her on and Enhance Her Orgasm 

You've tried everything and still can't get her in the mood? Not to worry. In my duty to provide men with the most accurate information on female sexuality, I decided to conduct a nation wide survey.


For people with disabilities, dating is even harder than it is for those with limited time in their schedule. Not only is it more difficult to get around for some, but there's also the risk of rejection that many disabled people fear when asking someone out on a date in person.
 




Using the intuition I had on types of pheromones, I thought that writing this article would indeed be worth the trouble. Most of the relevant information on types of pheromones has been included here.

As my body relaxed and my mind began to drift from stressful thoughts, I began to enjoy how great it felt to be touched. Because there was no pressure for sex, I was able to let my guard down. One thing led to another and soon enough we were both experiencing the hottest steamiest sex ever!

Your look is a very small part of your whole seduction package. A truly seductive woman has many tools in her arsenal. She knows how to get his interest and to keep it focused on her alone. She knows how to get a man to cross a room just to speak to her, and how to get him to call her that night. Before she leaves her home to go out, she is confident of how the evening will end. Part of this is confidence, gained from living the part of seduction diva, and part of it is the use of visualization techniques.The good news is that all of these techniques can be taught.

QUORUM SENSING EXPERIMENT NUMBER 1 In this present study, the producer and inducer strains were cross reacted to find out the production of antibiotic compound through quorum sensing. Totally three set of cultures were maintained as follows (along with one as control).

c) The normal 20 bacterial strains isolated from 20 algal species were crossed with terrestrial bacteria such as E-coli, Staphylococcus aureus separately

EXPERIMENT NUMBER 2 In this experiment, culture supernatant was obtained as per the procedure given in the experiment 1. 50ml of supernatant was mixed with equal volume of 80% methanol and 1% acetic acid mixture and it was shaked thoroughly in a separating funnel. Finally the methanol and acetic acid fractions were collected and concentrated by evaporation using water bath at 55??C. The viscous colloidal residues were resuspended in 600 microlitre of 50% methanol and it was used for antibacterial assay against different test organism.

3. Come Home Dirty and Sweaty Believe it or not, women love rugged looking men. There's something secretly sexy about dirty men. Brad Pitt, Harrison Ford, and Russel Crowe for instance, are irresistible to women because of their renegade appearance. Not only that, the scent of sweat is an aphrodisiac to women.

Holmstrom, C, D. Rittschof, and S. Kjelleberg. 1992. Inhibition of settlement by larvae of Balanus amphitrite by a surface - colonizing marine bacterium. Appl. Env. Microbiol. 58: 2111 -2115.

Bainton, N. J., P. Stead, and S.R. Chhabra. 1992. N-(3-oxohexanoyl)-L-homoserine Lactone regulated carbapenem antiobiotic production in Erwinia carotovora. Biochem. J. 288: 997-1004.

Disabled men and women want to be included in the fun and excitement of dating, too, without dear of incrimination from others in the online dating pool. The problem with a disabled person joining a website devoted exclusively to disabled dating is that the membership can be small.

When we allow ourselves these instincts kick in. Women automatically know to cross and uncross their legs, play with their hair, flirt with our eyes. No one took us aside and taught a class on it.

Bassler, B.L.1999. Curr.Opin.Microbiol.2: 582-587.* Bernan, V. S., M. Greenstein, and W. M. Maiese. 1997. Marine microorganisms as a source of new natural products. Adv. Appl. Microbiol. 43: 57 - 89.

In the gram negative bacteria AHSL is an active principle of quorum sensing. Our producer strain is also been identified as Pseudomonas sps. So in these organisms also active principle must falls under the AHSL. The cell-cell signaling mechanism can either require import of the signal and subsequent interaction with intracellular effectors or a two-component signaling system that transducers the information across the membrane. In V. harveyi genetic analysis of the density sensing apparatus has two independent density-sensing systems, and each is composed of a sensor-auto inducer pair; system one is composed of sensor I and Al -1, and system two is composed of sensor 2 and AI-2. The two densities - sensing system are redundant, because a null mutation in either system alone results in expression of hidden genes (Bassler, et al.,1999.).

There are things that you can do to be instantly more attractive to men. Some of these secrets involve the way you dress and apply your makeup, some involve the type of scent that you use and how you apply it and some involve more in depth seduction techniques and body language.

What does this mean? Let yourself get a little dirty and scruffy for your women. Fix her car with your shirt off or get hot and sweaty over a game of basketball. Either way the scent of sweat and the salty taste of your skin will drive her wild!

TEST ORGANISMS: 1. Epiphytic Vibrio from seaweeds 2. Vibrio from primary film 3. Vibrio from Sediments 4. Pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella sp. and Proteus sp

Table 2: The results of Quorum Sensing analysis of epibiotic bacterial isolates from seaweeds. Seaweed sample Producer organism Inducer organism Cross-species producer with inducer Cross-species supernatant test with inducer Zone of clearance (mm)

Boyd, K.G., A. Mearns -Soragg, G. Briedley, K. Hatzidimitriou, A. Rennie, M. Bregu, M. 0. Hubble, and J. G. Burgess. 1998. Antifouling potential of epiphytic marine bacteria from the surface of marine algae. Plouzane, France: 128 -136.

On an unconscious biological level we are given hidden attractors like pheromones and subtle physiological changes, like dilated pupils, the swelling of your lower lip and for men more obvious changes. We are also hard wired to know how to flirt.

Burgess, J. G., E. M. Jordan, M. Bregu, and A. Mearns-spragg. 1999. Microbial antagonism: a neglected avenue of natural products research. J. Biotechnology. 70: 27-32.

Cappuccino J. G., and N. Shermann. 1999. Microbiology, A Laboratory manual, Fourth edition, Addison Wesley, New York. Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998. The involvement of cell to cell signals in the development of a bacterial biofilm. Science. 280: 295-298.

- jewelry placed in eye catching areas ( ex. a carefully hung necklace to bring his eyes to your cleavage) - the hair toss - playing with your hair

Now, all my girlfriends are jealous of my sex life! - Carolina, SC 5. Listen to Reggaeton I'm from Puerto Rico, the home of Reggaeton, a new genre of music also hitting the United States. Reggaeton is a mix of Latin reggae and rap. The lyrics and rhythm of reggaeton songs are extremely sexual and erotic. The artists in these songs describe candidly what they want to do to women. Lyrics such as, "Te suelto el pelo, te quito la camisa, tu pantal??n y despu??s, te como completa," describe a scene where a man unties his woman's hair, takes off her shirt, her pants, and performs oral sex on her. Men should play reggaeton during sex to unleash wild fantasies in a woman's mind.

When my boyfriend and I come home after a day at the gym, we're so amped up; we have sex as soon as we walk through the door! - Becky, MA

The key to knowing how and when to use your attraction signals lies in examining your societies rules ( ex. interoffice romance is usually a no no) and working within their parameters.

Listening to reggaeton stimulates erotic scenes to play out in my mind. I'm left fantasizing about freaky wild sex and become very horny. Even if you're not Latin and don't understand the language, bringing a foreign element to the scene such as this is highly erotic to women. After all, Spanish is the language of love! - Raquel, PR

All the cultures were incubated at 28??C for 5 days. After the incubation period the cultures were centrifuged at 10,000 rpm for 15mins. The supernatant was collected and subjected to antibacterial assay with respective inducer strain.

In culture system B 200ul of 16 hours old producer strain alone was inoculated. In culture system C 200ul of 16 hours old inducer strain alone was inoculated.

Hiroaki, S., and K. M. Smith. 2003. Molecular mechanisms of bacterial quorum sensing as a new drug target. Curr. Opin. Chem. Biol. 7: 586-591. Hoang, T., Y. Ma, R. J. Stern, M. R. Meneil, and H. P. Schwezer. 1999. Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of Mycobacterium tuberculosis RmID and Pseudomonas aeruginosa Lasl and Rhll proteins, and functional analysis of purified Rhll. Gene. 237: 361 - 371.

In gram positive bacteria peptide and derivative peptide based signaling molecules seem to be the predominant mode of communication. During high cell density the marine bacteria can produce enzymes, surfactants, toxins, and antibiotics by the chemical signal communication. Marine epibiotic bacteria are also known to produce compounds active against drug resistant hospital pathogen by the cross species induction method. Building on assays described by Austin (Billaud and Austin 1990) a screening procedure has been developed in which marine bacteria are challenged by exposing them to terrestrial bacteria prior to assay of antimicrobial compounds. Hence in this present investigation it is proposed to find out the ability of sea weed epibiotic bacterial organism to produce antibacterial compounds through quorum sensing.

Microdictyon tenunis Chlorophyceae Chondrococcus hornemonii Chlorophyceae Enteromorpha intestinalis Chlorophyceae Caulerpa cupressoides Chlorophyceae

RESULTS AND DISCUSSION: QUORUM SENSING/CROSS SPECIES INDUCTION ANALYSIS In the present investigation totally 54 isolates were collected out of seaweed species. Among 54 isolates, 17 of them are producer strain, another 17 are the inducer strain rest of 20 isolates is normal and not showing any signs of activity (Table.2).

Do whatever it takes; the work definitely pays off! - Savita, Bombay On a closing note, I hope that I have delivered a better understanding into female sexuality. As you can see, simple things such as sending sexy text messages or letting yourself get scruffed up is all it takes for women to desire sex. It's not always about technique but about setting up an inviting atmosphere for sex and pleasure. Give these secrets a try and thank me later!

Every creature on this planet gives off attraction signals. We are no exception. Some are blatant and some are unconscious. We are all born with the ability to physically attract others. Some of us have just cultivated this gift.

Using the intuition I had on types of pheromones, I thought that writing this article would indeed be worth the trouble. Most of the relevant information on types of pheromones has been included here.



Icebreaker Pheromone Cologne



Starch hydrolysis test, Protein hydrolysis test, Lipid hydrolysis test, Oxidative / Fermentative test, Salt concentration (0%, 3%, 5%, 7%, 10%), TCBS, Growth in Temperature, 42??C and 47??C

Focusing on the senses is extremely arousing. Blindfolds are a great way for women to focus on his every touch and for men to learn exactly what pleases his women.

In the present investigation, it was aimed to produce the antibiotics from the seaweed epibionts through quorum sensing principle. The bacterial isolates of seaweed epibionts were identified as species of Pseudomonas and Vibrio from seaweeds Hypnea musiformis and Gracillaria edulis. In this study the Pseudomonas acts as a producer strain and Vibrio as inducer strain. The recent finding says that the seaweed epibionts having potential to control the metabolic activity of competitor organisms. Allison et al., 1998 reported that many bacterial strains up on attaching to a surface produce exopolysaccharides or exopolypeptides. In addition, it has been postulated that exopolysaccharides could mediate the attachment of the bacteria to the surface and induce metabolic changes.

Evelyn, A., Y. Liming, K.G. Boyd, P. C. Wright, and J. G. Burgess. 2001. The symbiotic role of marine microbes on living surfaces. Hydrobiologia. 461: 37 - 40

The earlier genetic analysis in Pseudomonas reveals the Pseudomonas consist of two quorum sensing systems as Las R1-I and Rh1R-l and have linked with R and I genes, in addition recently a third Lux R homolog that is advanced to a cluster of quorum sensing - controlled (qsc) genes were detected. Las R is a transcriptional regulator that responses primarily to the Las I - generated signal and Rh1R is a transcriptional regularly that responses best to the Rhl -generated signal. In Pseudomonas auriginosa, at low population densities Las I produce a basel level of 3-O-C12-HSL. As density increases, 3-0-C12-HSL builds to a critical concentration, at which point interacts with LasR. This Las R -3-0-012-HSL complex that activates transcription of a number of genes [Whileley, et al., 1999].

Hypnea valentiae Rhodophyceae Hypnea pannose Rhodophyceae Hypnea esperi Rhodophyceae Acanthophora spicifera Rhodophyceae ISOLATION OF EPIPHYTIC BACTERIA

Vandevivere, P., and D. L. Kirchman.1993. Attachment stimulates exopolysaccharide synthesis by a bacterium. Appl. Environ. Microbiol. 59: 3280-3286.

Attracting a man is never as difficult as we make it out to be. You are born with the knowledge of how to give off the right signals. Let go of your shyness, practice and suddenly you will realize how natural flirting and attracting men can be.

Mearns - Spragg, A., K. G. Boyd, and M. O. Hubble. 1997. Antibiotics from surface associated marine bacteria. Proceedings of the fourth underwater science symposium. The society for underwater Technology, London: 147 - 157.

Couples can invent their own games, complete with sexual penalties for losing as well! It's tantalizing fun! - Adele, Paris 7. Use Blindfolds

Because of different societal rules, many of our instincts have been suppressed. In certain cultures it is perfectly natural to walk around bare breasted, yet in others you can get arrested for this type of behavior.

Dictyota batryensis Phaeophyceae Sargassum sps Phaeophyceae Hypnea musciformis Rhodophyceae Acanthophora dendroides Rhodophyceae Jania rubens Rhodophyceae

All the above mentioned biochemical tests were performed by following standard methodology given in the Microbiological Laboratory Manual by James 3.Cappuccino (1999).

GcC- GcD- GcE- GcF- Gc A+/GcA- Gc B+/GcB- Gc C+/GcC- Gc D+/GcD- Gc E+ /GcE- Gc F+/GcF- GcA- GcB- GcC-

Vijaya baskar .P 1* AND Veera ravi.A 2 1. Department of biotechnology Dr. G.R.D.C.S, Coimbatore. 2. Department of biotechnology, Alagappa University, karaikudi.

Ines, M. M., B. Karaigher, U. Cepon, and I. Mahne. 2003. Variability of the Quorum sensing system in natural isolates of Bacillus sp., Food technol. Biotechnol. 41: 23 - 28.

Seaweeds itself secretes secondary metabolites to prevent fouling and grazing. In addition to that epibiotic bacteria on macro algae can also produce antifouling compounds that work in concert with the seaweed derived compounds to protect the seaweed surface. Recent studies have highlighted important roles of epibiotic bacteria colonizing the surface of seaweeds and releasing antifouling compounds. For the past 50 years antibiotics have revolutionized medicine by providing cures for formerly life threatening diseases. However, strains of bacteria have recently emerged that are virtually unresponsive to antibiotics such multidrug resistance, arising mainly through antibiotic misuse, is now recognized as a global health problem. In this situation, it is clear that new classes of antibiotics are urgently needed. Many marine bacteria have been shown to produce secondary metabolites that display antibacterial properties. The first antibiotic from a marine bacterium was identified and characterized in 1966. In addition, bacteria in biofilm on the surface of marine organisms have been documented to contain a higher proportion of antibiotic producing bacteria than some other marine environments (Burgess, et al., 1999). Marine epibiotic bacteria, associated with nutrient-rich algal surfaces have also been shown to produce antibacterial secondary metabolites which inhibit the settlement of potential competitors. Recently a lot new novel antibiotics such as Phenazine, thiomarinol, phenazine-1-carboxylic acid, 1-hydroxyphenazine 2-n-heptylquinol-4-one, 2-n-nonylquinol-4-one pyolipic, loloatins, agrochelin, sesbanimides, pelagiomicins, indomycione and indomycione have been identified from various marine epibiotic bacterial organisms. In particular, some species of the genus Pseudomonas produce both antibiotics and several other bioactive substances. For example, Pseudoalteromonas rubra and Pseudoalteromonas aurantia have been reported to be antibiotic producing bacteria. The phenomenon of higher organisms utilizing their associated microflora for the production of beneficial secondary metabolites is common in the marine environment (Yotsu, et al., 1987). A study of bacteria isolated from marine algae surfaces indicated that the incidence of antibiotic producing strains from this habitat was 20% whereas that from sea water was only a few percent. In addition, some bacteria that previously did not produce any active compounds have been found to be producing such metabolites when they are exposed to other bacterial species or extra cellular chemical from other bacteria. Bacteria may also produce antimicrobial compounds when they sense the presence of competing organisms. However, few attempts have been made to study such chemical communication between different bacterial species or how this might affect. The secretion of antimicrobial compounds (Mearns-Spragg, et al., 1998). Bacterial communication by the chemical signals for specific function is simply known as Quorum sensing. In which a bacterial population receives input from the environment and elicits an appropriate response (Hiroaki and Kristina. 2003). The term "quorum sensing" describes the ability of a microorganism to perceive and response to diffusible signal molecules. Bacterial cells sense their population density through a sophisticated cell to cell communication system and trigger expression of particular genes. Tne first system of density-dependent regulation was studied in detail with the luminescence of Photobacterium fischeri (formerly known as Vibrio fischeri) by Bassler et al., 1997. Eventually, they discovered that 3-oxo-N-(tetrahydro-2-oxo-3-furanyl) hexanamid or N-3-(oxohexanoyl) homoserine lactone (OHHL) was responsible the agent in the broth that induced luminescence. Followed by this many researchers have confirmed that in Gram negative bacteria acyl-homoserine lactone is responsible for the cell to cell communication system.

My boyfriend uses this technique to get me oh so horny! First, he gently lays me on the bed , tells me to relax, and close my eyes. He softly wraps the blindfold over my eyes; then begins to caress my skin with his fingertips. Since, my sense of sight is gone, the rest of my senses are heightened. His every touch feels exciting and erotic. He goes on to remove my clothes, one by one and kisses my bare skin as he moves along. I never know what he is going to do next or where he is going to touch. My favorite is when he when he dips his finger in sensitizing oil and strokes the outside of my vagina softly then firmly. This technique is just what it takes for me to beg him to give it to me! I recommend men using this technique as well. Once your fingers land on the right spot, your women will be throbbing with arousal; she'll beg you for sex! - Ariana, CO

6. Play with Sex Dice What truly turns me on is sex dice. If you haven't heard, sex dice is a set of regular dice but with sexual content on each face. For instance, there are sets that have one dice describing action words such as "lick, touch, tickle," and the other dice naming body parts such as "ass, tits, and lips." Sex dice come in many variations. There are some that describe a sexual position and a location to perform the act. Others are solely used for anal sex or oral sex. The list is endless. I personally own a set of just about each sex dice out there. I use them with my partners whenever I want to spice up the night. First, I'll start off slow and use a soft core sex dice. Then, as the night gets steamy, I bring out the freakier sex dice until we're engaging in the wildest sex ever! Since sex dice come in many variations, couples can find the right one for them. Whether your looking for some light fun or new ideas to spice up your sex life, these sex dice work wonders.


Zhang, L., P. J. Murphy, and A. Kerr. 1993. Agrobacterium conjugation and gene regulation by /V-acyl-L-homoserine lactones. Nature 362: 446 - 448.



This article has practically covered all points on types of pheromones. Do you feel the same thing upon reading this article?
Author Dave Thomson
 
 
 


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