The Benefits of Wearing Perfume 

Perfume has been used throughout history for a variety of reasons. Perfume has proved itself to be a valuable commodity in both ancient and modern times, with the modern perfume industry turnover in its billions every year. Perfume is a luxury, rather than a necessity, and yet its popularity grows rather than wavers every year. The reason behind this is the enjoyment that the wearer gains, and this type of enjoyment stems from the recognised and unnoticed benefits that wearing perfume provides.

If you find anything extra mentioning about human pheromone product, do inform us. It is only through the exchange of views and information will we learn more about human pheromone product.

Since the pheromones work on a subconscious level without the male even knowing it, it is a greater attracting force than your actions. It is a magnet working for you and if you know your man better you will be able to add to that bond of attraction, you have everything working for you and no negative factors. Hence pheromones work for you.

When you put on a pheromone cologne, you're not only restoring pheromones you've washed off, covered up or lost, but adding a chemical miracle substance that will work on a majority of women. By restoring and even enhancing your lost pheromones you can virtually "return to nature," and re-establish chemical communication with individuals around you. People react without even understanding why. They just feel they are magnetized to you.

EXPERIMENT NUMBER 2 In this experiment, culture supernatant was obtained as per the procedure given in the experiment 1. 50ml of supernatant was mixed with equal volume of 80% methanol and 1% acetic acid mixture and it was shaked thoroughly in a separating funnel. Finally the methanol and acetic acid fractions were collected and concentrated by evaporation using water bath at 55??C. The viscous colloidal residues were resuspended in 600 microlitre of 50% methanol and it was used for antibacterial assay against different test organism.

TEST ORGANISMS: 1. Epiphytic Vibrio from seaweeds 2. Vibrio from primary film 3. Vibrio from Sediments 4. Pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella sp. and Proteus sp

Also, be wary of what you're getting: make sure to maesure up package sizes when you purchase. Some manufacturers put as little as 10ml in a vial while charging a huge sum.

Burgess, J. G., M. Elizabeth, M. Bregu, A. Meams-Sprugg, and K. G. Boyd. 1999 Microbial antagonism: a neglected avenue of natural products research. J. Biotechnology. 70. 27-32.

The earlier genetic analysis in Pseudomonas reveals the Pseudomonas consist of two quorum sensing systems as Las R1-I and Rh1R-l and have linked with R and I genes, in addition recently a third Lux R homolog that is advanced to a cluster of quorum sensing - controlled (qsc) genes were detected. Las R is a transcriptional regulator that responses primarily to the Las I - generated signal and Rh1R is a transcriptional regularly that responses best to the Rhl -generated signal. In Pseudomonas auriginosa, at low population densities Las I produce a basel level of 3-O-C12-HSL. As density increases, 3-0-C12-HSL builds to a critical concentration, at which point interacts with LasR. This Las R -3-0-012-HSL complex that activates transcription of a number of genes [Whileley, et al., 1999].

Mckenney, D., K. E. Brown, and D. G. Allison. 1995. Influence of Pseudomonas aeruginosa exoproducts on virulence factor production in Burkholderia cepacia: evidence of interspecies communication. J. Bacterial 177: 6989 - 6992.

c) The normal 20 bacterial strains isolated from 20 algal species were crossed with terrestrial bacteria such as E-coli, Staphylococcus aureus separately

In the present investigation, it was aimed to produce the antibiotics from the seaweed epibionts through quorum sensing principle. The bacterial isolates of seaweed epibionts were identified as species of Pseudomonas and Vibrio from seaweeds Hypnea musiformis and Gracillaria edulis. In this study the Pseudomonas acts as a producer strain and Vibrio as inducer strain. The recent finding says that the seaweed epibionts having potential to control the metabolic activity of competitor organisms. Allison et al., 1998 reported that many bacterial strains up on attaching to a surface produce exopolysaccharides or exopolypeptides. In addition, it has been postulated that exopolysaccharides could mediate the attachment of the bacteria to the surface and induce metabolic changes.

Wagner, V. E., D. Bushneil, and L. Passador. 2003. Micro array analysis of Pseudomonas aeruginosa quorum sensing regulons. J. Bacteriol, 185: 2080 - 2095.

Human Pheromones The existence of human pheromones was found in 1986 and then onwards it has become an industry in itself. The manufacture of pheromones has become a multi million dollar business. The research and development in pheromones is also a booming business in USA.

Korber, D. R., J. R.Lawrence, H. M. Lappin-scott, and J. W. Costerton. 1995. Growth of microorganisms on surfaces. In. Microbial. Biofilm, Lapp in - Scott. Cambridge, UK: Cambridge university press.

If we possess our own pheromones, what is the reason we are in need of a pheromone cologne? Because of our cultural practices... What do we engage in first thing in the morning, every morning? Take a shower. Then we put on underarm roll-ons and perhaps lotions or aftershaves which further screen our natural body chemistry. Next, we tend to cover up from head to toe, thereby veiling ninety per cent of the very skin which releases pheromones into the air. A few researches have also revealed that via evolution, our natural pheromone production has gradually decreased, as civilizations have relied on social customs and norms when selecting a partner.

Pheromones are chemical aroma signals that all mammals/individuals emit. Subconsciously sensed, Nexus pheromones provide data about your immune system, your fertility, your present state of sexual interest and many more. A number of these pheromones focus on the opposite sex, and naturally trigger attraction arousal and enthusiasm for sex. With the proper chemical-hormonal signals, she spontaneously, intuitively responds without even knowing what is luring her to you!

Caulerpa racemosa Chlorophyceae Dictyota dichotoma Phaeophyceae Turbinaria ornata Phaeophyceae Padina gymnospora Phaeophyceae Sargassum cinearifolium Phaeophyceae

a) Among these 17 producers strain 6 strains were isolated from Hypnea musiformis. 6 from Gracillaria edulis, 4 from Ulva lactuca & 1 from Sediment.

We suggest that the above said mechanisms in Pseudomonas with quorum sensing principle might have occurred in the present study also. This induces the bacteria Pseudomonas in epibiotic seaweeds to secrete certain active compound against to the competitor Vibrio species.

Firstly, the effect of perfume on our brains is not necessarily recognised though the effects are certainly enjoyed. Each scent, whether natural or synthetic, emits molecules which enter the nasal passages and eventually reach the olfactory epithelium. This part of the nose has a vast amount of receptor neurons which are each covered in hairs called cilia. These cilia trap the scent and send a message to the brain which enables a person to recognise the perfume. The ability to smell immediately has a positive impact. This is important as some research has shown that people without a sense of smell have a less happy life than those who can smell; scents trigger emotions and affect our mood.

Vandevivere, P., and D. L. Kirchman.1993. Attachment stimulates exopolysaccharide synthesis by a bacterium. Appl. Environ. Microbiol. 59: 3280-3286.

Br B+ /Br B- Br C+/Br C- Br D+/Br D- Br E+/Br E- Br F+/Br F- BrA- Br B- BrC- BrD- BrE- BrF- NIL 39

Miller, M. B., and B. L. Bassler. 2001. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55: 165-199. Msadek, T. 1999. When the going gets tough: survival strategies and environmental signaling network in Bacillus subtilis. Trends Microbiol. 7: 201-207.

RESULTS AND DISCUSSION: QUORUM SENSING/CROSS SPECIES INDUCTION ANALYSIS In the present investigation totally 54 isolates were collected out of seaweed species. Among 54 isolates, 17 of them are producer strain, another 17 are the inducer strain rest of 20 isolates is normal and not showing any signs of activity (Table.2).

Bassler, B.L.1999. Curr.Opin.Microbiol.2: 582-587.* Bernan, V. S., M. Greenstein, and W. M. Maiese. 1997. Marine microorganisms as a source of new natural products. Adv. Appl. Microbiol. 43: 57 - 89.

All the cultures were incubated at 28??C for 5 days. After the incubation period the cultures were centrifuged at 10,000 rpm for 15mins. The supernatant was collected and subjected to antibacterial assay with respective inducer strain.

Bassler, B. L., E. P. Greenberg, and A. M. Sterens. 1997. Cross species induction of luminescence in the quorum - sensing bacterium Vibrio harveyi. J. Bacteriology. 179: 4043-4045.

Meains - Spragg, A., M. Bregu, K. G. Boyd, and J. G. Burgess. 1998. Cross species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Letters in Applied Microbiology. 27: 142-146.

Dictyota batryensis Phaeophyceae Sargassum sps Phaeophyceae Hypnea musciformis Rhodophyceae Acanthophora dendroides Rhodophyceae Jania rubens Rhodophyceae

How are pheromones sensed? Pheromone signals are sensed through an organ in the nose's interior named the Vomeronasal Organ (VNO). When the VNO detects the Nexus pheromone, it transmits a response signal to the brain. So, as the sense of smell is usually discussed in research regarding pheromone attraction, it is virtually a chemical response that occurs, instead of a specific identifiable smell. That is, she responds to your chemistry on an involuntary level, with feelings of attraction, stimulation, interest and enthusiasm.

ANTIBIOTIC ASSAYS Antibiotic activity was performed in duplicate using a standard paper disc diffusion method as well as well assay. In well assay 10mm in diameter wells were made in marine agar plates and the plates were swabbed with 16 hours old inducer strain. To these wells 200ul of cell free supernatant were added to each well. In paper disc assay the Watmann no.1 filter paper discs (6mm in diameter) were saturated with 200ul of cell free supernatant. The impregnant discs were Dlaced in the centre of the plates swabbed with test organisms. The plates were Incubated at 37??C overnight and observed for inhibition zone. The zone of inhibition was measured as the distance from the border of paper disc to the edge of the clear zone and expressed in mm.

Burgess, J. G., H. Miyashita, H. Sudo, and T. Matsunaga.1991. Antibiotic production by marine photosynthetic bacterium Chromatium purpuratum NKPB 031704: Localization of activity to the chromatophores. FEMS Microbiol. Lett. 84: 301 - 306.

Rheinheimer, G.1992.Aquatic microbiology (Wiley, New York), 3rd Ed. Stead, P., B. A. M. Rudd, H. Bradshaw, D. Nobel, and M. J. Dawson. 1996. Induction of phenazine biosynthesis in cultures Pseudomonas aeruginosa by L-N-(3-oxohexanoyl) homoserine lactone. FEMS Microbiology Letters 140: 15-22.

If you find anything extra mentioning about human pheromone product, do inform us. It is only through the exchange of views and information will we learn more about human pheromone product.

Icebreaker Pheromone Cologne

Vijaya baskar .P 1* AND Veera ravi.A 2 1. Department of biotechnology Dr. G.R.D.C.S, Coimbatore. 2. Department of biotechnology, Alagappa University, karaikudi.

As a result of this finding, perfume has become as much a science as it has an accessory. Aromatherapy studies the effects of natural botanical perfume ingredients, usually in the form of oils in their purest state. Its looks at the effects upon mood and general well being, and applies the knowledge by improving the perfume in the environment and offering a variety of aromatherapy services aimed at improving people`s lives. Aromacology applies this same approach to any available scent, from flowers to sand, and the findings are currently assisting perfume manufacturers worldwide.

The ingredients used in perfume may improve your concentration and enhance your mood. There is a long list of scents that are said to trigger this stimulating effect. Perfumes that contain ingredients such as ginger, sandalwood and rose are claimed by aromatherapists and aromacologists alike to have a stimulating effect on brain neurons. The increased stimulation aided by the use of perfume assists in many everyday tasks, from more focused studying to extra stamina and energy, balanced weight loss to an improved immune system.

Boyd, K.G., A. Mearns -Soragg, G. Briedley, K. Hatzidimitriou, A. Rennie, M. Bregu, M. 0. Hubble, and J. G. Burgess. 1998. Antifouling potential of epiphytic marine bacteria from the surface of marine algae. Plouzane, France: 128 -136.

All the above mentioned biochemical tests were performed by following standard methodology given in the Microbiological Laboratory Manual by James 3.Cappuccino (1999).

How Pheromones Are Used for Attracting Males In order to make a complete woman out of you and to competing with other women around your choice male, it might be necessary to get the attention of the male. The dressing is one way of doing it, but when you are in company of other females who are all similarly dressed, you might find that pheromones work on a subconscious level without your man knowing it. He is attracted to you by the action of pheromones and only to you. Make sure that your other actions do not impede the work of pheromones

Male Female Attraction through Pheromones The only way the lower living beings like ants, butterflies, birds, dogs or even a tiger can communicate is through pheromones. The pheromones are the way they communicate as they cannot communicate through oral or written word. The communication starts when the particular species it at its very best to procreate and ends when the species is not in a position to attract the opposite sex. Apart from the natural desire to find a mating partner, the pheromones also serve the purpose of communicating fight or flight signal.

Evelyn, A., Y. Liming, K.G. Boyd, P. C. Wright, and J. G. Burgess. 2001. The symbiotic role of marine microbes on living surfaces. Hydrobiologia. 461: 37 - 40

QUORUM SENSING EXPERIMENT NUMBER 1 In this present study, the producer and inducer strains were cross reacted to find out the production of antibiotic compound through quorum sensing. Totally three set of cultures were maintained as follows (along with one as control).

Quorum sensing usually focused on the bacteria growing in homogeneous environment. However few studies have attempted to a study this principle in heterogeneous environment also. In this present investigation we have attempted to study both homogeneous as well as heterogeneous environment. In former one we have isolated producer strain in seaweed eipbionts and it shows inhibitory activity against the inducer organism at the same seaweed epibionts. Later producer strains from seaweed epibionts, were treated with various Vibrio organisms from different environment. The obtain result of this study shows that the producer strain are capable of secreting antibiotic compounds not only to their natural competitors in its own habitate but also to the pathogen inhabiting in a distant related environment.

Seaweeds itself secretes secondary metabolites to prevent fouling and grazing. In addition to that epibiotic bacteria on macro algae can also produce antifouling compounds that work in concert with the seaweed derived compounds to protect the seaweed surface. Recent studies have highlighted important roles of epibiotic bacteria colonizing the surface of seaweeds and releasing antifouling compounds. For the past 50 years antibiotics have revolutionized medicine by providing cures for formerly life threatening diseases. However, strains of bacteria have recently emerged that are virtually unresponsive to antibiotics such multidrug resistance, arising mainly through antibiotic misuse, is now recognized as a global health problem. In this situation, it is clear that new classes of antibiotics are urgently needed. Many marine bacteria have been shown to produce secondary metabolites that display antibacterial properties. The first antibiotic from a marine bacterium was identified and characterized in 1966. In addition, bacteria in biofilm on the surface of marine organisms have been documented to contain a higher proportion of antibiotic producing bacteria than some other marine environments (Burgess, et al., 1999). Marine epibiotic bacteria, associated with nutrient-rich algal surfaces have also been shown to produce antibacterial secondary metabolites which inhibit the settlement of potential competitors. Recently a lot new novel antibiotics such as Phenazine, thiomarinol, phenazine-1-carboxylic acid, 1-hydroxyphenazine 2-n-heptylquinol-4-one, 2-n-nonylquinol-4-one pyolipic, loloatins, agrochelin, sesbanimides, pelagiomicins, indomycione and indomycione have been identified from various marine epibiotic bacterial organisms. In particular, some species of the genus Pseudomonas produce both antibiotics and several other bioactive substances. For example, Pseudoalteromonas rubra and Pseudoalteromonas aurantia have been reported to be antibiotic producing bacteria. The phenomenon of higher organisms utilizing their associated microflora for the production of beneficial secondary metabolites is common in the marine environment (Yotsu, et al., 1987). A study of bacteria isolated from marine algae surfaces indicated that the incidence of antibiotic producing strains from this habitat was 20% whereas that from sea water was only a few percent. In addition, some bacteria that previously did not produce any active compounds have been found to be producing such metabolites when they are exposed to other bacterial species or extra cellular chemical from other bacteria. Bacteria may also produce antimicrobial compounds when they sense the presence of competing organisms. However, few attempts have been made to study such chemical communication between different bacterial species or how this might affect. The secretion of antimicrobial compounds (Mearns-Spragg, et al., 1998). Bacterial communication by the chemical signals for specific function is simply known as Quorum sensing. In which a bacterial population receives input from the environment and elicits an appropriate response (Hiroaki and Kristina. 2003). The term "quorum sensing" describes the ability of a microorganism to perceive and response to diffusible signal molecules. Bacterial cells sense their population density through a sophisticated cell to cell communication system and trigger expression of particular genes. Tne first system of density-dependent regulation was studied in detail with the luminescence of Photobacterium fischeri (formerly known as Vibrio fischeri) by Bassler et al., 1997. Eventually, they discovered that 3-oxo-N-(tetrahydro-2-oxo-3-furanyl) hexanamid or N-3-(oxohexanoyl) homoserine lactone (OHHL) was responsible the agent in the broth that induced luminescence. Followed by this many researchers have confirmed that in Gram negative bacteria acyl-homoserine lactone is responsible for the cell to cell communication system.

The Best way to Wear Pheromone Femininity is the best pheromone and added to it the pheromone that you wear on your body; you have everything working for you. The best way to use the pheromone is to apply it on your breast and then allow the pheromones to work for you. Physical intimacy is not necessary but you have to be within just 2 to 7 feet for pheromones to be effective. The remote control of pheromones will make the male be attracted to you without his knowing it and remain attracted to you as long as you want it.

Another benefit to be gained from wearing perfume is that it can relax your mood. Certain scents have aldehydes which have a calming effect on the brain. The perfume oils which have this effect include eucalyptus and citronella. Other scents can act as a form of sedative, aiding restful sleep, which helps reduce anxiety and tension which in turn creates a wealth of health benefits. The most famous of these perfumes include lavender and bergamot, which have been used for this purpose for centuries and in a variety of countries worldwide.

CONCLUSION: The present investigation was aimed to study the quorum sensing principle among seaweed epibiotic bacterial organisms. In the past few decades there was no findings of new novel antibacterial class compounds were identified. But, the pathogenic microorganisms show much higher rate of resistant development even to the potential antibiotics. So, there is an urgent need to discover new novel antibiotic compounds. The marine inhabitants such as microorganisms, seaweeds, invertebrates, etc., act as an undepleted source of wide range of natural products among which the seaweeds act as a potential source of antibiotic compounds. Currently the cross species induction / quorum sensing attracts the total attention of researchers in finding new novel drugs against multidrug resistant pathogenic microorganisms. So, the present study aim to find out the capability of seaweed epibiotic bacterial organisms to produce novel drugs against the animal and plant pathogens

Wearing female pheromones is not an end all activity. Without the attendant personality and natural attraction it might not work well, but when you are competing with equals, wearing a pheromone will definitely give you the edge.

The aim of this article was to spread as much information on human pheromone product as possible. We surely do hope that we have succeeded in it.
Author Dave Thomson

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Pheromones are chemical aroma signals that all mammals/individuals emit. Subconsciously sensed, Nexus pheromones provide data about your immune system, your fertility, your present state of sexual interest and many more. A number of these pheromones focus on the opposite sex, and naturally trigger attraction arousal and enthusiasm for sex. With the proper chemical-hormonal signals, she...

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Many pheromone users try to mix two pheromone products, then fail, then go back to using standalone products. Here you can learn some basics facts about pheromone mixing. Naturally, it is much easier to create a pheromone mix if you know what you are mixing. The only way to know this is to use every pheromone product on its own first and see what reactions that pheromone product - cologne,...

Perfume has been used throughout history for a variety of reasons. Perfume has proved itself to be a valuable commodity in both ancient and modern times, with the modern perfume industry turnover in its billions every year. Perfume is a luxury, rather than a necessity, and yet its popularity grows rather than wavers every year. The reason behind this is the enjoyment that the wearer gains, and...

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