8/20/2017

Lubricants and oils, their role in love games 

A nice way for you too spice up your romantic night is by using massage oils to soothe those aching muscles. Aromatherapy oils are available which have a calming scent, massaged into your skin or applied to your bath water. Either way it will bring a smile to your face and leave you energized for a night of sexual pleasure. Some very erotic scents include forbidden fruit, lavender citrus and others. Heating oil is interesting and fun all in one, it is an oil for the more intimate massages. The heating oils heat on contact with the skin and grow hotter as you blow on it sending a feeling of warm pleasure coursing through the veins of the lucky recipient. Oils with pheromones and ginseng are also available to bring out your animal instinct. Massage oils come in varying sized bottles and are also available in kits including different kinds so you can find the right one for you and your partner.
 




Learning about things is what we are living here for now. So try to get to know as much about everything, including conquest pheromone whenever possible.


GcD- GcE- GcF- NIL NIL 26 NIL NIL NIL Ulva lactuca 13. U1+ 14: U2+ 15. U3+ 16. U4+ U1-

We suggest that the above said mechanisms in Pseudomonas with quorum sensing principle might have occurred in the present study also. This induces the bacteria Pseudomonas in epibiotic seaweeds to secrete certain active compound against to the competitor Vibrio species.

The collected seaweed samples were thoroughly washed with sterile seawater to removes the loosely attached bacteria/particles. Seaweed fronds were scrubbed with sterile cotton swabs to obtain epiphytic bacteria. Epiphytic bacterial organism in the swab were inoculated in sterile peptone broth (50% sea water) and incubated at 28??C in an incubated shaker (220 rpm / min) for overnight. After the incubation period the enriched cultures were serially diluted up to 10-8 concentration and 200 microlitre of each diluted samples were transferred into the nutrient agar plate (50% sea water). The plates were incubated at 28??C for 5 days and the plates with crowded colonies were selected. In the crowded plates those colonies, which showed the sign of inhibition zone around its margin to the neighboring colony, were selected and considered as producer strain. The neighboring sensitive colonies were treated as inducer strain. Both producer and inducer strains were streaked repeatedly until to get pure culture. The pure culture were properly labeled and subjected to the quorum sensing analysis.

Mearns - Spragg, A., K. G. Boyd, and M. O. Hubble. 1997. Antibiotics from surface associated marine bacteria. Proceedings of the fourth underwater science symposium. The society for underwater Technology, London: 147 - 157.

U2- U3- U4- U1+/U1- U2+/U2- U3+/U3- U4+/U4- U1- U2- U3- U4- NIL NIL NIL NIL Sediment 17. SA+ SA- SA+/SA- SA- 28

Personal lubricant can play a major role for a lot of people in sexual relations, whatever reason you may be prone to being dry, a personal lubricant can help. They come in water based and silicone based formulas, as well as a mixture of the two. Lubricants keep you slick and safe for a long night of passion. They will not leave a greasy residue nor do they stain or have an odor and they also clean up easy. Ingredients can include vitamin e and Aloe Vera. Most are water soluble and are safe with latex condoms. For hours of smooth safe fun, lubricants are just the thing. Personal lubricants come in bottles varying in size by ounces, some squeeze out the bottom while others use a pump for easier distribution.

Another benefit to be gained from wearing perfume is that it can relax your mood. Certain scents have aldehydes which have a calming effect on the brain. The perfume oils which have this effect include eucalyptus and citronella. Other scents can act as a form of sedative, aiding restful sleep, which helps reduce anxiety and tension which in turn creates a wealth of health benefits. The most famous of these perfumes include lavender and bergamot, which have been used for this purpose for centuries and in a variety of countries worldwide.

Patterson, G. L., and C. Ml. Bolis. 1997. Fungal cell - wall poly sacchraides elicit an antifungal secondary metabolite in the Cyanobacterium Scytonema ocellatum. J. Phycology. 33: 54 - 60.

All the 17 strains were named as PRODUCERS STAINS BrA+, BrB+, BrC+, BrD+, BrE+, BrF+ Hypnea musiformis GcA+, GcB+, GcC+, GcD+, GcE+, GcF+ Gracillaria edulis

Compliments are a simple but effective way to make a woman you are interested in feel special. However, most men don't know how to give good compliments. Here are just a few basic tips:

Liming, Y., G. K. Boyd, and J. G. Burgess. 2002. Surface induced attachment induced production of Antimicrobial compounds by marine epibiotic Bacteria using modified Roller Bottle. Mar. Biotechnology. 4: 356 - 366.

Dekievit, T. R., and B. H. Iglewski. 2000. Bacterial quorum sensing in pathogenic relationships. Infect. Immun. 66: 4839-4849. Dimango, E., H. J. Zar, R. Bryan, and A. Prince. 1995. Diverse Pseudomonas aerugniosa gene products stimulate respiratory epithelial cells to produce interleukin-8. J. Clin-lnvestig. 96: 2204-2210.

This article is under GNU FDL license and can be distributed without any previous authorization from the author. However the author's name and all the URLs (links) mentioned in the article and biography must be kept.

Bassler, B. L., E. P. Greenberg, and A. M. Sterens. 1997. Cross species induction of luminescence in the quorum - sensing bacterium Vibrio harveyi. J. Bacteriology. 179: 4043-4045.

Whiteley, M., K.M.Lee, and E.P.Greenberg.1999. Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa. Proc.Natl.Acad.Sci.USA.96:13904-13909.

Don't overuse compliments. If you compliment her too often, it will make you look like a pathetic wuss who is seeking approval. And it can be really annoying. For every compliment that you give her, tease her and poke fun at her insecurities at least twice. Be careful with this - a lady must know that you are just having fun with her.

In the present investigation, it was aimed to produce the antibiotics from the seaweed epibionts through quorum sensing principle. The bacterial isolates of seaweed epibionts were identified as species of Pseudomonas and Vibrio from seaweeds Hypnea musiformis and Gracillaria edulis. In this study the Pseudomonas acts as a producer strain and Vibrio as inducer strain. The recent finding says that the seaweed epibionts having potential to control the metabolic activity of competitor organisms. Allison et al., 1998 reported that many bacterial strains up on attaching to a surface produce exopolysaccharides or exopolypeptides. In addition, it has been postulated that exopolysaccharides could mediate the attachment of the bacteria to the surface and induce metabolic changes.

This experiment does not showed any inhibition zones Bacterial identification The 3 producer and 3 inducer strains which were responded the quorum sensing principles alone were subjected to biochemical analysis for identification. The obtained results revealed that all the producer strains showed sings of Pseudomonas sps and the inducer strains showed signs of Vibrio sps. So, based on the obtained result all the producer strains seems to be a Pseudomonas sps where as all the Inducer strain belongs to the genus vibrio.

RESULTS AND DISCUSSION: QUORUM SENSING/CROSS SPECIES INDUCTION ANALYSIS In the present investigation totally 54 isolates were collected out of seaweed species. Among 54 isolates, 17 of them are producer strain, another 17 are the inducer strain rest of 20 isolates is normal and not showing any signs of activity (Table.2).

Trischman, J.A., D.M.Tapiolas, P.R.Jensen, R.Dwight, W.Fenical, T.C.Mckee, C.M.Ireland, T.J.Stout, J.CIarely. 1994. Salinamide - A and salinamide - B - anti -inflammatory depsipeptides from a marine streptomycete. J.Am.Chem.Soc. 116: 757 -758.

If you and your loved one are looking for new and exciting ways of expressing your love for one another, these fore mentioned oils and lubricants can add just that. Impress your lover by treating them to a sensual massage, indulge in mutual foreplay with your choice in flavored lubricant, get out the safe latex friendly personal lubricant and have fun. All of the oil and lubricants are aimed at foreplay which is a critical part of love making; it raises the romance and can increase the desire you have for that lucky person.

In this present work the totally 54 isolates were screened from 20 different seaweed species out of which 34 species were showed the signs of quorum sensing i.e. 17 producer and 17 inducer strains, but when these organisms where subjected to quorum sensing principle in mixed culture only 3 them have responded. So the present study reveals around 17% of bacterial species isolated from seaweeds and sediment were responded to quorum sensing. According to the results of bacteria isolated from marine algae surfaces indicated that the incidence of antibiotic producing strains from this habitat was 20% where as that from sea water was only a few percent. In the present study also reveals more or less the same ratio in Pseudomonas spp. was observed. Our results also reveals a results of Kell et al., 1995; Stead et al., 1996; they have said the culture supernatant of Pseudomonas sps known to contain AHLS which induces the production of phenazine antibiotics. In this investigation due to time constraint, It was not attempted to identify the active compound secreted by Pseudomonas through quorum sensing, which may leave the space for the further intensive research in future.

Flavored lubricants can also add an element of fun to your planned evening of romance. Ingredients in these kinds of lubricants include, glycerin, water, natural flavoring, cellulose gum, are all very safe to be tasted. The flavors can provide you with a new level of enjoyment and includes tastes like passion fruit, watermelon, peach, apple, orange and many others. Most flavored lubricants will not stain, have no nasty after taste and are latex friendly. So be ready for a nice night of foreplay, just rub some on and lick it off, you will have fun and so will your lover. They come in bottles which pump or pour, or tubes which squeeze out the end. Just pick your favorite flavor, also like the oil they also come in variety packs which have small samplers of different flavors.

illaud, A. C, and B. Austin. 1990. Inhibition of the fish pathogen, Serratia liquefaciens by an antibiotic producing isolates of planococcus recovered from sea water. Journal of

Liming, Y., K. G. Boyd, and D. R. Adams. 2003. Biofilm specific cross species induction of antimicrobial compounds in Bacilli. AppI.Envi.Microbiol, 69:3719-3727.

The ingredients used in perfume may improve your concentration and enhance your mood. There is a long list of scents that are said to trigger this stimulating effect. Perfumes that contain ingredients such as ginger, sandalwood and rose are claimed by aromatherapists and aromacologists alike to have a stimulating effect on brain neurons. The increased stimulation aided by the use of perfume assists in many everyday tasks, from more focused studying to extra stamina and energy, balanced weight loss to an improved immune system.

In concluding this discussion, the quorum sensing is wider spread among bacterial population then was previously thought, (In Gram positive, Gram negative bacterial communication). Current assays for antimicrobial activities are inadequate because some antibiotic producing bacteria may require the presence of another bacterial species. These findings have important implication for the discovery of novel antimicrobial compounds from marine bacteria and may allow the development of new methods for screening novel compounds active against multidrug resistant bacteria.

Learning about things is what we are living here for now. So try to get to know as much about everything, including conquest pheromone whenever possible.



Icebreaker Pheromone Cologne



QUORUM SENSING EXPERIMENT NUMBER 1 In this present study, the producer and inducer strains were cross reacted to find out the production of antibiotic compound through quorum sensing. Totally three set of cultures were maintained as follows (along with one as control).

Yotsu, T., Y.Meguro, A. Endo, M. Murate, H. Naold, and T. Yasumoto.1987. Production of tetrodotoxin and its derivatives by Pseudomonas sp. Toxicon 25: 225 - 228

Table 2: The results of Quorum Sensing analysis of epibiotic bacterial isolates from seaweeds. Seaweed sample Producer organism Inducer organism Cross-species producer with inducer Cross-species supernatant test with inducer Zone of clearance (mm)

Caulerpa racemosa Chlorophyceae Dictyota dichotoma Phaeophyceae Turbinaria ornata Phaeophyceae Padina gymnospora Phaeophyceae Sargassum cinearifolium Phaeophyceae

*Corresponding author e-mail: vijay10bas@yahoo.co.in INTRODUCTION We are living in a microbial planet. About 71 % of the surface of this planet is covered by sea water. A typical milliliter of seawater contains 103 fungal cells, 106 bacteria, and 107 viruses, including pathogens that cause widespread mortalities and microbes that initiate fouling of host surfaces (Rheinheimer, 1992). Thus, marine plants and animals are continually exposed to high concentrations of potentially harmful microbes. These microorganisms in nature exists as free living planktonic mode of life in sea water or it may exist as epibiotic organisms in various living and nonliving surfaces. Among living organisms, seaweeds and invertebrates act as suitable substrate for the establishment of epibiotic organisms Seaweeds are known to release a large amount of organic carbon into the surrounding environment providing a nutrient rich habitat for microorganisms like bacteria. Bacteria are generally considered to be independent unicellular organisms. One cell accomplishes all of the tasks of feeding, locomotion, 'reproduction, respiration and all other processes necessary to keep an organism alive. There are several classes of bacteria such as primary film forming bacteria, sediment bacteria, symbiotic bacteria, and epibiotic bacteria in various aquatic organisms. The marine surface environment is a site of intense composition for living space by a wide variety of organisms. Bacteria are generally recognized as primary colonizers of this habitat and are able to form biofilm on marine surface such as invertebrates and algae (Bryers, et al., 1982). Bacteria may also be abundant on the surfaces of some algae as an important epibiotic organism. In many cases, the bacterial population found to be specific, with changes occurring throughout the year or life span of the algal surface. This algal-bacterial relationship is symbiotic in most cases; the epibiotic bacteria in seaweed play a protective role by releasing secondary metabolites into the surrounding seawater that help preventing extensive fouling of the surface. Epibiotic bacteria are therefore attracting attention as a source of new natural products. Bacteria from the larvae of some crustaceans protect them from fungal infection by the production of simple antimicrobial compounds. Bacteria isolated from the surface of a tunicate prevented the settlement of barnacle and tunicate larvae exposed to the bacteria as biofilm in petridishes (Evelyn et al., 2001).

Holmstrom, C, and S. Kjelleberg. 1994. The effect of external biological factors on, settlement of marine invertebrates and new antifouling technologies. Biofouling. 8: 147-160.

GcA-, GcB-, GcC-, GcD- GcE-, GcF- Gracillaria edulis U1-, U2-, U3-, U4- Ulva lactuca SA- Sediment

Firstly, the effect of perfume on our brains is not necessarily recognised though the effects are certainly enjoyed. Each scent, whether natural or synthetic, emits molecules which enter the nasal passages and eventually reach the olfactory epithelium. This part of the nose has a vast amount of receptor neurons which are each covered in hairs called cilia. These cilia trap the scent and send a message to the brain which enables a person to recognise the perfume. The ability to smell immediately has a positive impact. This is important as some research has shown that people without a sense of smell have a less happy life than those who can smell; scents trigger emotions and affect our mood.

Armstrong, E., J. D. Mckenzie, and G.T. G. Worthy. 1999. Aquaculture of sponges on scallops for natural products research and antifouling. J. Biotechnol. 70: 163-174. 70. 21-

Meains - Spragg, A., M. Bregu, K. G. Boyd, and J. G. Burgess. 1998. Cross species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Letters in Applied Microbiology. 27: 142-146.

BACTERIAL IDENTIFICATION The organisms responded to the quorum sensing process alone were identified by the following biochemical analysis.

The earlier genetic analysis in Pseudomonas reveals the Pseudomonas consist of two quorum sensing systems as Las R1-I and Rh1R-l and have linked with R and I genes, in addition recently a third Lux R homolog that is advanced to a cluster of quorum sensing - controlled (qsc) genes were detected. Las R is a transcriptional regulator that responses primarily to the Las I - generated signal and Rh1R is a transcriptional regularly that responses best to the Rhl -generated signal. In Pseudomonas auriginosa, at low population densities Las I produce a basel level of 3-O-C12-HSL. As density increases, 3-0-C12-HSL builds to a critical concentration, at which point interacts with LasR. This Las R -3-0-012-HSL complex that activates transcription of a number of genes [Whileley, et al., 1999].

Give a compliment that reflects the effect that she has on you. This kind of compliment emphasizes the relationship between the two of you, rather than just the things she does.

a) Among these 17 producers strain 6 strains were isolated from Hypnea musiformis. 6 from Gracillaria edulis, 4 from Ulva lactuca & 1 from Sediment.

U1+, U2+, U3+, U4+ Ulva lactuca SA+ Sediment INDUCER STRAIN BrA-, BrB-, BrC-, BrD-, BrE-, BrF- Hypnea musiformis

All the above mentioned biochemical tests were performed by following standard methodology given in the Microbiological Laboratory Manual by James 3.Cappuccino (1999).

In gram positive bacteria peptide and derivative peptide based signaling molecules seem to be the predominant mode of communication. During high cell density the marine bacteria can produce enzymes, surfactants, toxins, and antibiotics by the chemical signal communication. Marine epibiotic bacteria are also known to produce compounds active against drug resistant hospital pathogen by the cross species induction method. Building on assays described by Austin (Billaud and Austin 1990) a screening procedure has been developed in which marine bacteria are challenged by exposing them to terrestrial bacteria prior to assay of antimicrobial compounds. Hence in this present investigation it is proposed to find out the ability of sea weed epibiotic bacterial organism to produce antibacterial compounds through quorum sensing.

Starch hydrolysis test, Protein hydrolysis test, Lipid hydrolysis test, Oxidative / Fermentative test, Salt concentration (0%, 3%, 5%, 7%, 10%), TCBS, Growth in Temperature, 42??C and 47??C

Holmstrom, C, D. Rittschof, and S. Kjelleberg. 1992. Inhibition of settlement by larvae of Balanus amphitrite by a surface - colonizing marine bacterium. Appl. Env. Microbiol. 58: 2111 -2115.

The test organisms Vibrio species were isolated from seaweed as epiphyles, biofilm, sediment and puffer fish by using TCBS medium (Hi media) The pathogenic bacteria were collected from clinical laboratories.

ABSTRACT Bacterial biofilms are sessile communities with high cell density that are ubiquitous in natural, medical, and engineering environments; they are fascinating since they are primitive tissues with an advanced chemical communications network. Currently, there is an explosive amount of biofilm research, most of it with the ultimate aims of biofilm prevention, control, or eradication.The present investigation was aimed to study the cell signaling principle among seaweed epibiotic bacterial organisms , totally 54 bacterial isolates were made out of 20 seaweed species. Among 54 bacterial isolates 17 of them producer, another 17 of them inducer strains rest of 20 isolates were normal that have not showing any signs of activity. The 17 producers and 17 inducer strains were subjected to the cross species induction by quorum sensing principle and found that only 3 of them have responded to the cross species induction in production of antibacterial compound against the respective inducer strains. All the 3 producers and inducer were identified by biochemical analysis and surprisingly that all the 3 producer strain belongs to Genus Pseudomonas and inducer strains were belongs to Genus Vibrio. The supernatant obtain from mixed culture of Pseudomonas & Vibrio shows the antibiotic activity against the various Vibrio species which were isolated from other sources. Such as Vibrio from primary film, Vibrio from sediment Vibrio from seaweeds epibiotics. The obtain results clearly shows the particular Pseudomonas from seaweed epibionts has the capability of producing potential antibiotic compound against wide range of Vibrio species through quorum sensing.


Give compliments that you sincerely believe to be true. Keep the compliments short and sweet. Speak in a friendly voice and don't sound boring. Make eye contact and smile. Make sure your smile is not a fake smile - just relax your face and you'll have a natural warm smile.



We had never known how interesting writing about conquest pheromone would be, until we got to write this article. Hope you felt the same too.
Author Dave Thomson
 
 
 


coolgne and pheromones | conquest pheromone | date matep heromones | define pheromone | date mate pheromones | cornell university pheromone study | dark continent pheromone |

Lean More about "conquest pheromone"

To put it bluntlyIm going to be frank, nobody likes being dumped and few of us take pleasure in being the "dumper" either. The end of any relationship, often inspite of its length, will be a traumatic time for both parties involved where your emotions run rampant. Understanding, compassion, patience and especially, honesty, are the key factors in any successful break up, if there even...

Opposites attract is a law of attraction, at least where electromagnetism is concerned. But are there laws about attraction between two people? In a world that is full of strangers as a line in a famous song of the 1980 s goes, is there a clear set of rules that allows two people to fall for each other? Is attraction a matter of chemistry? Maybe. According to scientists, the...

Compliments are a simple but effective way to make a woman you are interested in feel special. However, most men don t know how to give good compliments. Here are just a few basic tips: Try to give a unique compliment about a certain characteristic that most people overlook. For example, never tell her how beautiful she is, because everyone has said that to her. Compliment a woman for her...

Does he like me? is an age old question. If you weren t so busy being insecure you would know the answer in seconds. You can tell if a guy likes your girlfriends can t you? Take a step back and notice the signs. Does he actually look into your eyes? Is he interested in what you have to say? Does he rearrange his schedule to make sure that he can see you? Hmm all good signs. Does he put...

Is there a difference between pheromone perfume and cologne? What should you consider when shopping for human pheromone products? There really isn t any difference at all. Cologne is generally targeted towards men while perfume is basically a woman s product. It s simply marketing as women would identify much more with pheromone perfume. Spray and concentrate products then complete the...

Sex on the first date? Oh, here is a nice taboo topic that I know everyone talks about, with their friends and a few choice people. But few people talk about it out in the open. So the big question is really, yes or no, do we have sex on the first date or not? Well to tell you the truth, it s all a personal opinion. What if the sexual attraction is there between you and the other person? We know...

Tongat Ali is an herbal supplement that is used as a sexual stimulant. Never heard of it? Don t feel bad you are in good company; it is still not widely known about. Tongat Ali, like yohimbine is a plant based sexual stimulant and like the Yohimbe plant the Tongat Ali is becoming hard to find especially since it is now protected from harvest by law in Malaysia which is the original source of...

Everyone likes romance, don t they? When we think of a romantic date we conjure up images of moonlight, fine wine, flowers, chocolates, candlelight dinners and dancing close under the stars. Are there ways to help keep that romance and magic alive as a relationship progresses? Certainly there are. Let us take a look at ways to do just that. Establish a secret word or a secret phrase between...





pheromone??
sexanemal
Europe and the United States women mate and animal images
7
Dog control woman Videos
animalsexwomen
animal meeting sex
which is world best strong pheromones attract the women
Free Xxx Video Of Women Having Sex With Horses
androstenol Bt

 
 
 
 
Copyright (c) 2007 by gilroypheromones.com - All Rights Reserved | Site Map | RSS Feed | Conquest Pheromone - Lubricants and oils, their role in love games