10/23/2018

10 Ways to Turn Her on and Enhance Her Orgasm 

You've tried everything and still can't get her in the mood? Not to worry. In my duty to provide men with the most accurate information on female sexuality, I decided to conduct a nation wide survey.


Boyd, K.G., A. Mearns -Soragg, G. Briedley, K. Hatzidimitriou, A. Rennie, M. Bregu, M. 0. Hubble, and J. G. Burgess. 1998. Antifouling potential of epiphytic marine bacteria from the surface of marine algae. Plouzane, France: 128 -136.
 




Writing an article on monarch butterfly pheromones was our foremost priority while thinking of a topic to write on. This is because monarch butterfly pheromones are interesting parts of our lives, and are needed by us.

MATERIALS AND METHODS: SAMPLE COLLECTION: Seaweed samples were collected from Gulf of Mannar Marine Biosphere Reserve and identified up to species level by using CMFRI bulletin (14) as follows:

Table 2: The results of Quorum Sensing analysis of epibiotic bacterial isolates from seaweeds. Seaweed sample Producer organism Inducer organism Cross-species producer with inducer Cross-species supernatant test with inducer Zone of clearance (mm)

b) Among these 17-inducer strain 6 strains were isolated from Hypnea musiformis, 6 from Gracillaria edulis, 4 from Ulva lactuca & 1 from sediment.

U1+, U2+, U3+, U4+ Ulva lactuca SA+ Sediment INDUCER STRAIN BrA-, BrB-, BrC-, BrD-, BrE-, BrF- Hypnea musiformis

RESULTS AND DISCUSSION: QUORUM SENSING/CROSS SPECIES INDUCTION ANALYSIS In the present investigation totally 54 isolates were collected out of seaweed species. Among 54 isolates, 17 of them are producer strain, another 17 are the inducer strain rest of 20 isolates is normal and not showing any signs of activity (Table.2).

So should I stop trying to become attractive? More than trying to become physically attractive, work on all aspects of your health: physical, emotional, mental, and spiritual. Physical attraction is still a precursor. Remember, biology predisposes us to choose the partner with the healthiest genes. Where your emotions are concerned, just ask this to yourself: would you want to spend time with a person who feels insecure about him/herself?

Caulerpa racemosa Chlorophyceae Dictyota dichotoma Phaeophyceae Turbinaria ornata Phaeophyceae Padina gymnospora Phaeophyceae Sargassum cinearifolium Phaeophyceae

"Opposites attract" is a law of attraction, at least where electromagnetism is concerned. But are there laws about attraction between two people? "In a world that is full of strangers" as a line in a famous song of the 1980's goes, is there a clear set of rules that allows two people to fall for each other?

Human pheromones are natural chemicals produced by the body that are thought to cause the attraction between opposite sexes. While it is believed that there is some connection, the real analysis lies in how much do human pheromones and sexual attraction depend on each other.

illaud, A. C, and B. Austin. 1990. Inhibition of the fish pathogen, Serratia liquefaciens by an antibiotic producing isolates of planococcus recovered from sea water. Journal of

According to James V. Kohl, coauthor of "The Scent of Eros," women are led by their sense of smell when it comes to sexual attraction. They are especially receptive at detecting pheromones, a special aromatic chemical released by humans to sexually attract one another. Another study, conducted by Bern University known as the "Stinky T-shirt Study" concluded that women were attracted to the pheromone chemicals left behind by men's sweat.

BACTERIAL IDENTIFICATION The organisms responded to the quorum sensing process alone were identified by the following biochemical analysis.

Vandevivere, P., and D. L. Kirchman.1993. Attachment stimulates exopolysaccharide synthesis by a bacterium. Appl. Environ. Microbiol. 59: 3280-3286.

8. Take her Dancing Many men aren't aware of the sexuality in dancing. While, romantic dinners can lead to the bedroom there is nothing more arousing than a night out dancing. When women are dancing, they begin to think of their body as sexy and graceful. Their self confidence increases and are no longer shy to expose it later on during the night. Dancing is like an ego booster for women. A woman can let loose, dress sexy, and feel good about herself. Not to mention, the atmosphere in a club, is less intimidating and proper than a fancy restaurant. I'm not saying to skip the fancy restaurant; in fact it's crucial to show women that they deserve to be treated with respect and class. However, a night of dancing helps jump start the sexual chemistry between two people.

6. Play with Sex Dice What truly turns me on is sex dice. If you haven't heard, sex dice is a set of regular dice but with sexual content on each face. For instance, there are sets that have one dice describing action words such as "lick, touch, tickle," and the other dice naming body parts such as "ass, tits, and lips." Sex dice come in many variations. There are some that describe a sexual position and a location to perform the act. Others are solely used for anal sex or oral sex. The list is endless. I personally own a set of just about each sex dice out there. I use them with my partners whenever I want to spice up the night. First, I'll start off slow and use a soft core sex dice. Then, as the night gets steamy, I bring out the freakier sex dice until we're engaging in the wildest sex ever! Since sex dice come in many variations, couples can find the right one for them. Whether your looking for some light fun or new ideas to spice up your sex life, these sex dice work wonders.

Rheinheimer, G.1992.Aquatic microbiology (Wiley, New York), 3rd Ed. Stead, P., B. A. M. Rudd, H. Bradshaw, D. Nobel, and M. J. Dawson. 1996. Induction of phenazine biosynthesis in cultures Pseudomonas aeruginosa by L-N-(3-oxohexanoyl) homoserine lactone. FEMS Microbiology Letters 140: 15-22.

Bainton, N. J., P. Stead, and S.R. Chhabra. 1992. N-(3-oxohexanoyl)-L-homoserine Lactone regulated carbapenem antiobiotic production in Erwinia carotovora. Biochem. J. 288: 997-1004.

Okay, it wasn't exactly a nation-wide survey. I contacted my female friends (most are straight) and asked them, "What truly turns you on?" So now you're privy to all their naughtiest secrets.

CONCLUSION: The present investigation was aimed to study the quorum sensing principle among seaweed epibiotic bacterial organisms. In the past few decades there was no findings of new novel antibacterial class compounds were identified. But, the pathogenic microorganisms show much higher rate of resistant development even to the potential antibiotics. So, there is an urgent need to discover new novel antibiotic compounds. The marine inhabitants such as microorganisms, seaweeds, invertebrates, etc., act as an undepleted source of wide range of natural products among which the seaweeds act as a potential source of antibiotic compounds. Currently the cross species induction / quorum sensing attracts the total attention of researchers in finding new novel drugs against multidrug resistant pathogenic microorganisms. So, the present study aim to find out the capability of seaweed epibiotic bacterial organisms to produce novel drugs against the animal and plant pathogens

In the gram negative bacteria AHSL is an active principle of quorum sensing. Our producer strain is also been identified as Pseudomonas sps. So in these organisms also active principle must falls under the AHSL. The cell-cell signaling mechanism can either require import of the signal and subsequent interaction with intracellular effectors or a two-component signaling system that transducers the information across the membrane. In V. harveyi genetic analysis of the density sensing apparatus has two independent density-sensing systems, and each is composed of a sensor-auto inducer pair; system one is composed of sensor I and Al -1, and system two is composed of sensor 2 and AI-2. The two densities - sensing system are redundant, because a null mutation in either system alone results in expression of hidden genes (Bassler, et al.,1999.).

Mearns - Spragg, A., K. G. Boyd, and M. O. Hubble. 1997. Antibiotics from surface associated marine bacteria. Proceedings of the fourth underwater science symposium. The society for underwater Technology, London: 147 - 157.

My boyfriend uses this technique to get me oh so horny! First, he gently lays me on the bed , tells me to relax, and close my eyes. He softly wraps the blindfold over my eyes; then begins to caress my skin with his fingertips. Since, my sense of sight is gone, the rest of my senses are heightened. His every touch feels exciting and erotic. He goes on to remove my clothes, one by one and kisses my bare skin as he moves along. I never know what he is going to do next or where he is going to touch. My favorite is when he when he dips his finger in sensitizing oil and strokes the outside of my vagina softly then firmly. This technique is just what it takes for me to beg him to give it to me! I recommend men using this technique as well. Once your fingers land on the right spot, your women will be throbbing with arousal; she'll beg you for sex! - Ariana, CO

Ines, M. M., B. Karaigher, U. Cepon, and I. Mahne. 2003. Variability of the Quorum sensing system in natural isolates of Bacillus sp., Food technol. Biotechnol. 41: 23 - 28.

Evelyn, A., Y. Liming, K.G. Boyd, P. C. Wright, and J. G. Burgess. 2001. The symbiotic role of marine microbes on living surfaces. Hydrobiologia. 461: 37 - 40

Yotsu, T., Y.Meguro, A. Endo, M. Murate, H. Naold, and T. Yasumoto.1987. Production of tetrodotoxin and its derivatives by Pseudomonas sp. Toxicon 25: 225 - 228

3. Come Home Dirty and Sweaty Believe it or not, women love rugged looking men. There's something secretly sexy about dirty men. Brad Pitt, Harrison Ford, and Russel Crowe for instance, are irresistible to women because of their renegade appearance. Not only that, the scent of sweat is an aphrodisiac to women.

All the cultures were incubated at 28??C for 5 days. After the incubation period the cultures were centrifuged at 10,000 rpm for 15mins. The supernatant was collected and subjected to antibacterial assay with respective inducer strain.

Trischman, J.A., D.M.Tapiolas, P.R.Jensen, R.Dwight, W.Fenical, T.C.Mckee, C.M.Ireland, T.J.Stout, J.CIarely. 1994. Salinamide - A and salinamide - B - anti -inflammatory depsipeptides from a marine streptomycete. J.Am.Chem.Soc. 116: 757 -758.

This experiment does not showed any inhibition zones Bacterial identification The 3 producer and 3 inducer strains which were responded the quorum sensing principles alone were subjected to biochemical analysis for identification. The obtained results revealed that all the producer strains showed sings of Pseudomonas sps and the inducer strains showed signs of Vibrio sps. So, based on the obtained result all the producer strains seems to be a Pseudomonas sps where as all the Inducer strain belongs to the genus vibrio.

Writing an article on monarch butterfly pheromones was our foremost priority while thinking of a topic to write on. This is because monarch butterfly pheromones are interesting parts of our lives, and are needed by us.



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What did it for me was his incredible ass massage. The extra attention to groping, rubbing, and squeezing my ass, got my blood flowing in all the right places. It was just what a needed to experience an intense orgasm later. - Marisa, CT

Once you've developed a strong bond with one another, she'll be looking forward to showing you a sneak peak of her wild side. The club is just the right place for it! - Natalia, Moscow

Hypnea valentiae Rhodophyceae Hypnea pannose Rhodophyceae Hypnea esperi Rhodophyceae Acanthophora spicifera Rhodophyceae ISOLATION OF EPIPHYTIC BACTERIA

4. Talk Dirty Talking dirty was once intimidating to me, now it's essential to my sex life. In the past, my girlfriend's competed with one another about which boyfriend said the freakiest thing during sex. I use to be very jealous and wished my sex life was as exciting as theirs. One day, I asked them how they became comfortable with talking dirty and how it was introduced in their relationship. Based on their advice, here's what worked for me and what I suggest for couples.

First, fantasize about having sex with your partner and talking dirty to them. Soon enough, your thoughts will be expressed out loud. Start out by describing what feels good during sex. For example, you can say, "I love the way your... feels in my..." Describe what movements feel good to your body or what you're going to do to your partner. If your at a loss for words, research sexy erotica novels for a list of words. Remember, there is no right or wrong way to talk dirty. Don't try to imitate movies; be yourself. This will be more comfortable for the both of you. Once you have the right environment to talk dirty, sex is exciting and extremely passionate.

Now, all my girlfriends are jealous of my sex life! - Carolina, SC 5. Listen to Reggaeton I'm from Puerto Rico, the home of Reggaeton, a new genre of music also hitting the United States. Reggaeton is a mix of Latin reggae and rap. The lyrics and rhythm of reggaeton songs are extremely sexual and erotic. The artists in these songs describe candidly what they want to do to women. Lyrics such as, "Te suelto el pelo, te quito la camisa, tu pantal??n y despu??s, te como completa," describe a scene where a man unties his woman's hair, takes off her shirt, her pants, and performs oral sex on her. Men should play reggaeton during sex to unleash wild fantasies in a woman's mind.

Hiroaki, S., and K. M. Smith. 2003. Molecular mechanisms of bacterial quorum sensing as a new drug target. Curr. Opin. Chem. Biol. 7: 586-591. Hoang, T., Y. Ma, R. J. Stern, M. R. Meneil, and H. P. Schwezer. 1999. Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of Mycobacterium tuberculosis RmID and Pseudomonas aeruginosa Lasl and Rhll proteins, and functional analysis of purified Rhll. Gene. 237: 361 - 371.

Liming, Y., G. K. Boyd, and J. G. Burgess. 2002. Surface induced attachment induced production of Antimicrobial compounds by marine epibiotic Bacteria using modified Roller Bottle. Mar. Biotechnology. 4: 356 - 366.

Bassler, B. L., E. P. Greenberg, and A. M. Sterens. 1997. Cross species induction of luminescence in the quorum - sensing bacterium Vibrio harveyi. J. Bacteriology. 179: 4043-4045.

Korber, D. R., J. R.Lawrence, H. M. Lappin-scott, and J. W. Costerton. 1995. Growth of microorganisms on surfaces. In. Microbial. Biofilm, Lapp in - Scott. Cambridge, UK: Cambridge university press.

Since there are still some unexplored aspects of human pheromones, there are also different opinions on its effectiveness. Some scientists do not believe pheromones are as important in humans as they are in other species.

QUORUM SENSING EXPERIMENT NUMBER 1 In this present study, the producer and inducer strains were cross reacted to find out the production of antibiotic compound through quorum sensing. Totally three set of cultures were maintained as follows (along with one as control).

According to the Journal of Sex and Marital Therapy, researcher Rosemary Basson, MD concludes that women must convince themselves to want sex before their sexual desire kicks in. Many women agree, including myself. Arousal starts first in the mind. Give your woman sexy thoughts throughout the day to stir her sexual desire. Send her sexy text messages or emails while she's at work. Include little reminders from when you had great sex. Tell her what you want to do to her once she gets home. If your hesitant and are afraid she'll be offended, start off with a fun flirty text message. My personal favorites are from www.lingo2word.com. There you'll find hot text messages such as "I love every bone in your body, including mine!" These sexy messages are just what I need to start thinking about sex and becoming aroused throughout my day.

Miller, M. B., and B. L. Bassler. 2001. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55: 165-199. Msadek, T. 1999. When the going gets tough: survival strategies and environmental signaling network in Bacillus subtilis. Trends Microbiol. 7: 201-207.

GcD- GcE- GcF- NIL NIL 26 NIL NIL NIL Ulva lactuca 13. U1+ 14: U2+ 15. U3+ 16. U4+ U1-

As a women with a lot on my plate (work, kids, husband) my sex drive was at an all time low. In fact, it was to the point that my husband basically gave up trying. One night, I came home with a body ache and asked my husband to give me a massage. Little did we know we were going to be in for a treat!

c) The normal 20 bacterial strains isolated from 20 algal species were crossed with terrestrial bacteria such as E-coli, Staphylococcus aureus separately

Scientists have determined a marked difference in the pheromones between males and females. It has also been looked at as to why there are different pheromones for each gender.

Kell, D. B., A. S. Kaprelyants, and A. Grafen. 1995. Pheromones, Social behaviour and the functions of secondary metabolism in bacteria. Trends in Ecol. and Evol. 10: 126-129.

REFERENCES Alim, I., and K. Yuto. 2003. Mc21-A, a Bacteriocidal antibiotic produced by a new marine bacterium, Pseudoalteromonas phenotica sp. nov. O-Bc 30T, against Methicillin -Resistant Staphylococcus aureus. Antimicro. Agents Chemoth. 47: 480 - 488.

Is attraction a matter of chemistry? Maybe. According to scientists, the attraction between animals of the opposite sex is all about chemicals called pheromones. The effect of pheromones in behavior of insects is the most studied to date. It has been observed, at least in some experiments, that pheromones are responsible for communication among same species and colony of ants. The horrible odor released by skunks to ward off enemies is said to be a kind of pheromone. Some species of apes rub pheromone-containing urine on the feet of potential mates to attract them.

Lately, scientists are looking into the existence of human pheromones and its role in mate selection. There are many conflicting views in the realm of biology, chemistry, genetics, and psychology. Most scientists would assert that these do not exist, or if they do, do not play a role in sexual attraction between a man and a woman. But new researches such as that conducted by Swiss researchers from the University of Bern led by Klaus Wedekind are slowly making these scientists rethink their stand. Their experiment involved women sniffing the cotton shirts of different men during their ovulation period. It was found out that women prefer the smell of men's shirts that were genetically different, but also shared similarities with the women's genes. This, like in the case of insects and other mammals, was to ensure better and healthier characteristics for their future children. But researchers also cautioned that preference for a male odor is affected by:

- The women's ovulation period - The food that men eat - Perfumes and other scented body products - And lastly the use of contraceptive pills.

The collected seaweed samples were thoroughly washed with sterile seawater to removes the loosely attached bacteria/particles. Seaweed fronds were scrubbed with sterile cotton swabs to obtain epiphytic bacteria. Epiphytic bacterial organism in the swab were inoculated in sterile peptone broth (50% sea water) and incubated at 28??C in an incubated shaker (220 rpm / min) for overnight. After the incubation period the enriched cultures were serially diluted up to 10-8 concentration and 200 microlitre of each diluted samples were transferred into the nutrient agar plate (50% sea water). The plates were incubated at 28??C for 5 days and the plates with crowded colonies were selected. In the crowded plates those colonies, which showed the sign of inhibition zone around its margin to the neighboring colony, were selected and considered as producer strain. The neighboring sensitive colonies were treated as inducer strain. Both producer and inducer strains were streaked repeatedly until to get pure culture. The pure culture were properly labeled and subjected to the quorum sensing analysis.

Probably not! There is wisdom in knowing yourself: who you are, your beliefs, values, and dreams. And do not pretend to be someone you are not. Fooling another person by making him/her think that you share the same values and beliefs is only going to cause you both disappointments. When you are healthy in all aspects, attractiveness becomes a consequence and not an end. As mentioned in the Klohnen and Luo's research, a person's sense of self-security matters, perhaps even beyond attraction. But remember: do these things for yourself and not for other people. Only then can you truly harness your attractiveness as a person.



This is our humble presentation on monarch butterfly pheromones. Your reading it will add the necessary weightage to the presentation.
Author Dave Thomson
 
 
 


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