10/18/2017

The Benefits of Wearing Perfume 

Perfume has been used throughout history for a variety of reasons. Perfume has proved itself to be a valuable commodity in both ancient and modern times, with the modern perfume industry turnover in its billions every year. Perfume is a luxury, rather than a necessity, and yet its popularity grows rather than wavers every year. The reason behind this is the enjoyment that the wearer gains, and this type of enjoyment stems from the recognised and unnoticed benefits that wearing perfume provides.
 




There are universal applications on human hperomone product everywhere. However, it is up to us to decide the way used for these applications to get the best results from them.


As a result of this finding, perfume has become as much a science as it has an accessory. Aromatherapy studies the effects of natural botanical perfume ingredients, usually in the form of oils in their purest state. Its looks at the effects upon mood and general well being, and applies the knowledge by improving the perfume in the environment and offering a variety of aromatherapy services aimed at improving people`s lives. Aromacology applies this same approach to any available scent, from flowers to sand, and the findings are currently assisting perfume manufacturers worldwide.

Male Female Attraction through Pheromones The only way the lower living beings like ants, butterflies, birds, dogs or even a tiger can communicate is through pheromones. The pheromones are the way they communicate as they cannot communicate through oral or written word. The communication starts when the particular species it at its very best to procreate and ends when the species is not in a position to attract the opposite sex. Apart from the natural desire to find a mating partner, the pheromones also serve the purpose of communicating fight or flight signal.

Armstrong, E., J. D. Mckenzie, and G.T. G. Worthy. 1999. Aquaculture of sponges on scallops for natural products research and antifouling. J. Biotechnol. 70: 163-174. 70. 21-

Liming, Y., K. G. Boyd, and D. R. Adams. 2003. Biofilm specific cross species induction of antimicrobial compounds in Bacilli. AppI.Envi.Microbiol, 69:3719-3727.

Bainton, N. J., P. Stead, and S.R. Chhabra. 1992. N-(3-oxohexanoyl)-L-homoserine Lactone regulated carbapenem antiobiotic production in Erwinia carotovora. Biochem. J. 288: 997-1004.

All the above mentioned biochemical tests were performed by following standard methodology given in the Microbiological Laboratory Manual by James 3.Cappuccino (1999).

Are there other ways to heighten my sexual libido?

Of course, the most favored way to enhance any part of your health is to do away with unfavorable habits such as smoking and habitual drinking, give yourself a better diet and exercise daily. While you're developing healthier living overall, adding a male enhancement supplement can take you to an even more sought-after level of sexual performance and enjoyment.

All the cultures were incubated at 28??C for 5 days. After the incubation period the cultures were centrifuged at 10,000 rpm for 15mins. The supernatant was collected and subjected to antibacterial assay with respective inducer strain.

Whiteley, M., K.M.Lee, and E.P.Greenberg.1999. Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa. Proc.Natl.Acad.Sci.USA.96:13904-13909.

Ines, M. M., B. Karaigher, U. Cepon, and I. Mahne. 2003. Variability of the Quorum sensing system in natural isolates of Bacillus sp., Food technol. Biotechnol. 41: 23 - 28.

GcA-, GcB-, GcC-, GcD- GcE-, GcF- Gracillaria edulis U1-, U2-, U3-, U4- Ulva lactuca SA- Sediment

Quorum sensing usually focused on the bacteria growing in homogeneous environment. However few studies have attempted to a study this principle in heterogeneous environment also. In this present investigation we have attempted to study both homogeneous as well as heterogeneous environment. In former one we have isolated producer strain in seaweed eipbionts and it shows inhibitory activity against the inducer organism at the same seaweed epibionts. Later producer strains from seaweed epibionts, were treated with various Vibrio organisms from different environment. The obtain result of this study shows that the producer strain are capable of secreting antibiotic compounds not only to their natural competitors in its own habitate but also to the pathogen inhabiting in a distant related environment.

Starch hydrolysis test, Protein hydrolysis test, Lipid hydrolysis test, Oxidative / Fermentative test, Salt concentration (0%, 3%, 5%, 7%, 10%), TCBS, Growth in Temperature, 42??C and 47??C

ABSTRACT Bacterial biofilms are sessile communities with high cell density that are ubiquitous in natural, medical, and engineering environments; they are fascinating since they are primitive tissues with an advanced chemical communications network. Currently, there is an explosive amount of biofilm research, most of it with the ultimate aims of biofilm prevention, control, or eradication.The present investigation was aimed to study the cell signaling principle among seaweed epibiotic bacterial organisms , totally 54 bacterial isolates were made out of 20 seaweed species. Among 54 bacterial isolates 17 of them producer, another 17 of them inducer strains rest of 20 isolates were normal that have not showing any signs of activity. The 17 producers and 17 inducer strains were subjected to the cross species induction by quorum sensing principle and found that only 3 of them have responded to the cross species induction in production of antibacterial compound against the respective inducer strains. All the 3 producers and inducer were identified by biochemical analysis and surprisingly that all the 3 producer strain belongs to Genus Pseudomonas and inducer strains were belongs to Genus Vibrio. The supernatant obtain from mixed culture of Pseudomonas & Vibrio shows the antibiotic activity against the various Vibrio species which were isolated from other sources. Such as Vibrio from primary film, Vibrio from sediment Vibrio from seaweeds epibiotics. The obtain results clearly shows the particular Pseudomonas from seaweed epibionts has the capability of producing potential antibiotic compound against wide range of Vibrio species through quorum sensing.

BACTERIAL IDENTIFICATION The organisms responded to the quorum sensing process alone were identified by the following biochemical analysis.

How long do Pheromones work? This varies a lot depending on each person's unique chemistry and the particular scent they pick. One of the best products, Nexus Pheromones, avers its pheromone-based cologne could last for an estimated 8-10 hours when spread on the right places.

The earlier genetic analysis in Pseudomonas reveals the Pseudomonas consist of two quorum sensing systems as Las R1-I and Rh1R-l and have linked with R and I genes, in addition recently a third Lux R homolog that is advanced to a cluster of quorum sensing - controlled (qsc) genes were detected. Las R is a transcriptional regulator that responses primarily to the Las I - generated signal and Rh1R is a transcriptional regularly that responses best to the Rhl -generated signal. In Pseudomonas auriginosa, at low population densities Las I produce a basel level of 3-O-C12-HSL. As density increases, 3-0-C12-HSL builds to a critical concentration, at which point interacts with LasR. This Las R -3-0-012-HSL complex that activates transcription of a number of genes [Whileley, et al., 1999].

The results of Vanderivere and Kirchman 1993 suggest that the addition of increased surface by adding sand will induce the exopolymer synthesis through the high cell density dependent system. In the same way the bacterial organisms attached to the surface of seaweed shows alteration of genes expression may be due to the response to the high competitive environment. When cell density increases the competition for space and nutrients are also increased. So the existing bacteria were forced to protect themselves in this competitive environment. Normally in this condition the bacteria will be activated to induce the expression of certain hidden genes in genetic material through quorum sensing. The quorum sensing is principles were active compounds (autoinducer) from bacterial cell will promote the expression of a particular hidden gene of other bacterial organism in a stressed condition.

Table 2: The results of Quorum Sensing analysis of epibiotic bacterial isolates from seaweeds. Seaweed sample Producer organism Inducer organism Cross-species producer with inducer Cross-species supernatant test with inducer Zone of clearance (mm)

Kell, D. B., A. S. Kaprelyants, and A. Grafen. 1995. Pheromones, Social behaviour and the functions of secondary metabolism in bacteria. Trends in Ecol. and Evol. 10: 126-129.

Bryers, J. D., and W. G. Characklis. 1982. Processes governing primary biofilm formation. Bioeng. 24: 2451-2476 Burgess, J. G., K. Boyd, E. Armstrong, T. Pisacane, and D. R. Adams. 2003. The development of a marine natural product-based antifouling paint. Biofouling. 19: 197 -205.

Evelyn, A., Y. Liming, K.G. Boyd, P. C. Wright, and J. G. Burgess. 2001. The symbiotic role of marine microbes on living surfaces. Hydrobiologia. 461: 37 - 40

Miller, M. B., and B. L. Bassler. 2001. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55: 165-199. Msadek, T. 1999. When the going gets tough: survival strategies and environmental signaling network in Bacillus subtilis. Trends Microbiol. 7: 201-207.

In culture system B 200ul of 16 hours old producer strain alone was inoculated. In culture system C 200ul of 16 hours old inducer strain alone was inoculated.

Caulerpa racemosa Chlorophyceae Dictyota dichotoma Phaeophyceae Turbinaria ornata Phaeophyceae Padina gymnospora Phaeophyceae Sargassum cinearifolium Phaeophyceae

Also, be wary of what you're getting: make sure to maesure up package sizes when you purchase. Some manufacturers put as little as 10ml in a vial while charging a huge sum.

Liming, Y., G. K. Boyd, and J. G. Burgess. 2002. Surface induced attachment induced production of Antimicrobial compounds by marine epibiotic Bacteria using modified Roller Bottle. Mar. Biotechnology. 4: 356 - 366.

Microdictyon tenunis Chlorophyceae Chondrococcus hornemonii Chlorophyceae Enteromorpha intestinalis Chlorophyceae Caulerpa cupressoides Chlorophyceae

Hypnea valentiae Rhodophyceae Hypnea pannose Rhodophyceae Hypnea esperi Rhodophyceae Acanthophora spicifera Rhodophyceae ISOLATION OF EPIPHYTIC BACTERIA

U1+, U2+, U3+, U4+ Ulva lactuca SA+ Sediment INDUCER STRAIN BrA-, BrB-, BrC-, BrD-, BrE-, BrF- Hypnea musiformis

How Pheromones Are Used for Attracting Males In order to make a complete woman out of you and to competing with other women around your choice male, it might be necessary to get the attention of the male. The dressing is one way of doing it, but when you are in company of other females who are all similarly dressed, you might find that pheromones work on a subconscious level without your man knowing it. He is attracted to you by the action of pheromones and only to you. Make sure that your other actions do not impede the work of pheromones

There are universal applications on human hperomone product everywhere. However, it is up to us to decide the way used for these applications to get the best results from them.



Icebreaker Pheromone Cologne



ANTIBIOTIC ASSAYS Antibiotic activity was performed in duplicate using a standard paper disc diffusion method as well as well assay. In well assay 10mm in diameter wells were made in marine agar plates and the plates were swabbed with 16 hours old inducer strain. To these wells 200ul of cell free supernatant were added to each well. In paper disc assay the Watmann no.1 filter paper discs (6mm in diameter) were saturated with 200ul of cell free supernatant. The impregnant discs were Dlaced in the centre of the plates swabbed with test organisms. The plates were Incubated at 37??C overnight and observed for inhibition zone. The zone of inhibition was measured as the distance from the border of paper disc to the edge of the clear zone and expressed in mm.

Cappuccino J. G., and N. Shermann. 1999. Microbiology, A Laboratory manual, Fourth edition, Addison Wesley, New York. Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998. The involvement of cell to cell signals in the development of a bacterial biofilm. Science. 280: 295-298.

GcC- GcD- GcE- GcF- Gc A+/GcA- Gc B+/GcB- Gc C+/GcC- Gc D+/GcD- Gc E+ /GcE- Gc F+/GcF- GcA- GcB- GcC-

illaud, A. C, and B. Austin. 1990. Inhibition of the fish pathogen, Serratia liquefaciens by an antibiotic producing isolates of planococcus recovered from sea water. Journal of

Holmstrom, C, D. Rittschof, and S. Kjelleberg. 1992. Inhibition of settlement by larvae of Balanus amphitrite by a surface - colonizing marine bacterium. Appl. Env. Microbiol. 58: 2111 -2115.

Selective Pheromones Males are attracted to female pheromones. While male pheromones have been given the name of androstenone, no such name could be found to the female pheromones. It is referred by the same name and marketed through the brand names. In the world dominated by the males, where men are trying to woo females, it might be difficult to find a female trying to woo just any male. Women have to be specific and thus should be trying to woo a particular male by using a female pheromone.

Bassler, B. L., E. P. Greenberg, and A. M. Sterens. 1997. Cross species induction of luminescence in the quorum - sensing bacterium Vibrio harveyi. J. Bacteriology. 179: 4043-4045.

TEST ORGANISMS: 1. Epiphytic Vibrio from seaweeds 2. Vibrio from primary film 3. Vibrio from Sediments 4. Pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella sp. and Proteus sp

Mearns - Spragg, A., K. G. Boyd, and M. O. Hubble. 1997. Antibiotics from surface associated marine bacteria. Proceedings of the fourth underwater science symposium. The society for underwater Technology, London: 147 - 157.

What is the reason for pheromone products? This may be the thing that offers you the competitive edge and the self-assurance you need to reach your social and sexual objectives, whatever they may be. Pheromones are a simple way to boost your attractiveness, and then match it with the confidence you'll have from all of the choices and interest.

Burgess, J. G., H. Miyashita, H. Sudo, and T. Matsunaga.1991. Antibiotic production by marine photosynthetic bacterium Chromatium purpuratum NKPB 031704: Localization of activity to the chromatophores. FEMS Microbiol. Lett. 84: 301 - 306.

Are all pheromone products about the same? No, definitely not. They're all completely different, and the wise consumer must always shop warily. You should scout for a product which has pure androstenone Nexus pheromone concentrate especially in "double strength" potency. If you are having trouble searching for the appropriate product, we suggest you consider Nexus Pheromones.

In this present work the totally 54 isolates were screened from 20 different seaweed species out of which 34 species were showed the signs of quorum sensing i.e. 17 producer and 17 inducer strains, but when these organisms where subjected to quorum sensing principle in mixed culture only 3 them have responded. So the present study reveals around 17% of bacterial species isolated from seaweeds and sediment were responded to quorum sensing. According to the results of bacteria isolated from marine algae surfaces indicated that the incidence of antibiotic producing strains from this habitat was 20% where as that from sea water was only a few percent. In the present study also reveals more or less the same ratio in Pseudomonas spp. was observed. Our results also reveals a results of Kell et al., 1995; Stead et al., 1996; they have said the culture supernatant of Pseudomonas sps known to contain AHLS which induces the production of phenazine antibiotics. In this investigation due to time constraint, It was not attempted to identify the active compound secreted by Pseudomonas through quorum sensing, which may leave the space for the further intensive research in future.

Yotsu, T., Y.Meguro, A. Endo, M. Murate, H. Naold, and T. Yasumoto.1987. Production of tetrodotoxin and its derivatives by Pseudomonas sp. Toxicon 25: 225 - 228

Why is it that I haven't learned of about this fascinating new sense organ and human pheromones before? Because it's a recent discovery! Although you may not have known about pheromones and the sixth sense until this time, you will: They're swiftly becoming common words.

Zhang, L., P. J. Murphy, and A. Kerr. 1993. Agrobacterium conjugation and gene regulation by /V-acyl-L-homoserine lactones. Nature 362: 446 - 448.

Do we emit pheromones by nature? Without question, we are chemically conversing with each other constantly. These pheromones that we emit are the basic ingredient for many pheromone cologne type products.

EXPERIMENT NUMBER 2 In this experiment, culture supernatant was obtained as per the procedure given in the experiment 1. 50ml of supernatant was mixed with equal volume of 80% methanol and 1% acetic acid mixture and it was shaked thoroughly in a separating funnel. Finally the methanol and acetic acid fractions were collected and concentrated by evaporation using water bath at 55??C. The viscous colloidal residues were resuspended in 600 microlitre of 50% methanol and it was used for antibacterial assay against different test organism.

Burgess, J. G., E. M. Jordan, M. Bregu, and A. Mearns-spragg. 1999. Microbial antagonism: a neglected avenue of natural products research. J. Biotechnology. 70: 27-32.

Holmstrom, C, and S. Kjelleberg. 1994. The effect of external biological factors on, settlement of marine invertebrates and new antifouling technologies. Biofouling. 8: 147-160.

What effects must I anticipate with a pheromone cologne? A marked increase in eye contact, interest and smiles from the opposite sex Increased sex appeal More dates and lovemaking A restoration of your existing relationship More ardent lovemaking A soothing, effortless effect on people around you More instances of being approached by the opposite sex Improved self confidence Spontaneous respect and attention on you by others.

In the present investigation, it was aimed to produce the antibiotics from the seaweed epibionts through quorum sensing principle. The bacterial isolates of seaweed epibionts were identified as species of Pseudomonas and Vibrio from seaweeds Hypnea musiformis and Gracillaria edulis. In this study the Pseudomonas acts as a producer strain and Vibrio as inducer strain. The recent finding says that the seaweed epibionts having potential to control the metabolic activity of competitor organisms. Allison et al., 1998 reported that many bacterial strains up on attaching to a surface produce exopolysaccharides or exopolypeptides. In addition, it has been postulated that exopolysaccharides could mediate the attachment of the bacteria to the surface and induce metabolic changes.

c) The normal 20 bacterial strains isolated from 20 algal species were crossed with terrestrial bacteria such as E-coli, Staphylococcus aureus separately

U2- U3- U4- U1+/U1- U2+/U2- U3+/U3- U4+/U4- U1- U2- U3- U4- NIL NIL NIL NIL Sediment 17. SA+ SA- SA+/SA- SA- 28

Dictyota batryensis Phaeophyceae Sargassum sps Phaeophyceae Hypnea musciformis Rhodophyceae Acanthophora dendroides Rhodophyceae Jania rubens Rhodophyceae

Pheromones are chemical aroma signals that all mammals/individuals emit. Subconsciously sensed, Nexus pheromones provide data about your immune system, your fertility, your present state of sexual interest and many more. A number of these pheromones focus on the opposite sex, and naturally trigger attraction arousal and enthusiasm for sex. With the proper chemical-hormonal signals, she spontaneously, intuitively responds without even knowing what is luring her to you!

QUORUM SENSING EXPERIMENT NUMBER 1 In this present study, the producer and inducer strains were cross reacted to find out the production of antibiotic compound through quorum sensing. Totally three set of cultures were maintained as follows (along with one as control).

In the gram negative bacteria AHSL is an active principle of quorum sensing. Our producer strain is also been identified as Pseudomonas sps. So in these organisms also active principle must falls under the AHSL. The cell-cell signaling mechanism can either require import of the signal and subsequent interaction with intracellular effectors or a two-component signaling system that transducers the information across the membrane. In V. harveyi genetic analysis of the density sensing apparatus has two independent density-sensing systems, and each is composed of a sensor-auto inducer pair; system one is composed of sensor I and Al -1, and system two is composed of sensor 2 and AI-2. The two densities - sensing system are redundant, because a null mutation in either system alone results in expression of hidden genes (Bassler, et al.,1999.).


Wearing female pheromones is not an end all activity. Without the attendant personality and natural attraction it might not work well, but when you are competing with equals, wearing a pheromone will definitely give you the edge.



We had thought that producing some matter on human hperomone product would be an impossibility. However, once we started, there was no turning back.
Author Dave Thomson
 
 
 


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Perfume has been used throughout history for a variety of reasons. Perfume has proved itself to be a valuable commodity in both ancient and modern times, with the modern perfume industry turnover in its billions every year. Perfume is a luxury, rather than a necessity, and yet its popularity grows rather than wavers every year. The reason behind this is the enjoyment that the wearer gains, and...

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