11/11/2019

Do you Swing? Check Out Swingers and Swing Clubs Online! 

How do you feel about swinging? Do you consider that such sexual experiences can offer you and your partner some fresh perspective on sexuality-related issues? If you still are not certain if swinging is the best way to go, then read the following article. It will certainly offer you some perspective, plus mention the importance of online swinging communities and where to find swingers ads.


First, fantasize about having sex with your partner and talking dirty to them. Soon enough, your thoughts will be expressed out loud. Start out by describing what feels good during sex. For example, you can say, "I love the way your... feels in my..." Describe what movements feel good to your body or what you're going to do to your partner. If your at a loss for words, research sexy erotica novels for a list of words. Remember, there is no right or wrong way to talk dirty. Don't try to imitate movies; be yourself. This will be more comfortable for the both of you. Once you have the right environment to talk dirty, sex is exciting and extremely passionate.
 




Did you ever believe that there was so much to learn about pheromone pest control? Neither did we! Once we got to write this article, it seemed to be endless.

Cappuccino J. G., and N. Shermann. 1999. Microbiology, A Laboratory manual, Fourth edition, Addison Wesley, New York. Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998. The involvement of cell to cell signals in the development of a bacterial biofilm. Science. 280: 295-298.

Miller, M. B., and B. L. Bassler. 2001. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55: 165-199. Msadek, T. 1999. When the going gets tough: survival strategies and environmental signaling network in Bacillus subtilis. Trends Microbiol. 7: 201-207.

According to James V. Kohl, coauthor of "The Scent of Eros," women are led by their sense of smell when it comes to sexual attraction. They are especially receptive at detecting pheromones, a special aromatic chemical released by humans to sexually attract one another. Another study, conducted by Bern University known as the "Stinky T-shirt Study" concluded that women were attracted to the pheromone chemicals left behind by men's sweat.

All the cultures were incubated at 28??C for 5 days. After the incubation period the cultures were centrifuged at 10,000 rpm for 15mins. The supernatant was collected and subjected to antibacterial assay with respective inducer strain.

Allison, D. G., B. Ruiz, C. Sanjose, A. Jaspe, and P.Gilbert. 1998. Extracellular products as mediators of the formation and detachment of Pseudomonas fluorescens biofilms. FEMS Microbial Lett. 167: 179-184.

Trischman, J.A., D.M.Tapiolas, P.R.Jensen, R.Dwight, W.Fenical, T.C.Mckee, C.M.Ireland, T.J.Stout, J.CIarely. 1994. Salinamide - A and salinamide - B - anti -inflammatory depsipeptides from a marine streptomycete. J.Am.Chem.Soc. 116: 757 -758.

Dictyota batryensis Phaeophyceae Sargassum sps Phaeophyceae Hypnea musciformis Rhodophyceae Acanthophora dendroides Rhodophyceae Jania rubens Rhodophyceae

Bassler, B.L.1999. Curr.Opin.Microbiol.2: 582-587.* Bernan, V. S., M. Greenstein, and W. M. Maiese. 1997. Marine microorganisms as a source of new natural products. Adv. Appl. Microbiol. 43: 57 - 89.

Swinging is mainly the experiencing of something different. It is about the skin, the smell and the touch of a completely new person against yours. It is all about breaking stereotypes and transforming sexual fantasies into true realities. Swingers are not interested in making their sexual preferences known to the whole world; they maintain complete discretion and prefer to use the Internet to find people who can fulfill their desires.

Wagner, V. E., D. Bushneil, and L. Passador. 2003. Micro array analysis of Pseudomonas aeruginosa quorum sensing regulons. J. Bacteriol, 185: 2080 - 2095.

Vijaya baskar .P 1* AND Veera ravi.A 2 1. Department of biotechnology Dr. G.R.D.C.S, Coimbatore. 2. Department of biotechnology, Alagappa University, karaikudi.

NIL NIL NIL NIL Gracillaria edulis 7. GcA+ 8. GcB+ 9. GcC+ 10. GcD+ 11. GcE+ 12. GcF+ GcA- GcB-

Microdictyon tenunis Chlorophyceae Chondrococcus hornemonii Chlorophyceae Enteromorpha intestinalis Chlorophyceae Caulerpa cupressoides Chlorophyceae

The results of Vanderivere and Kirchman 1993 suggest that the addition of increased surface by adding sand will induce the exopolymer synthesis through the high cell density dependent system. In the same way the bacterial organisms attached to the surface of seaweed shows alteration of genes expression may be due to the response to the high competitive environment. When cell density increases the competition for space and nutrients are also increased. So the existing bacteria were forced to protect themselves in this competitive environment. Normally in this condition the bacteria will be activated to induce the expression of certain hidden genes in genetic material through quorum sensing. The quorum sensing is principles were active compounds (autoinducer) from bacterial cell will promote the expression of a particular hidden gene of other bacterial organism in a stressed condition.

Whiteley, M., K.M.Lee, and E.P.Greenberg.1999. Identification of genes controlled by quorum sensing in Pseudomonas aeruginosa. Proc.Natl.Acad.Sci.USA.96:13904-13909.

As a women with a lot on my plate (work, kids, husband) my sex drive was at an all time low. In fact, it was to the point that my husband basically gave up trying. One night, I came home with a body ache and asked my husband to give me a massage. Little did we know we were going to be in for a treat!

Seaweeds itself secretes secondary metabolites to prevent fouling and grazing. In addition to that epibiotic bacteria on macro algae can also produce antifouling compounds that work in concert with the seaweed derived compounds to protect the seaweed surface. Recent studies have highlighted important roles of epibiotic bacteria colonizing the surface of seaweeds and releasing antifouling compounds. For the past 50 years antibiotics have revolutionized medicine by providing cures for formerly life threatening diseases. However, strains of bacteria have recently emerged that are virtually unresponsive to antibiotics such multidrug resistance, arising mainly through antibiotic misuse, is now recognized as a global health problem. In this situation, it is clear that new classes of antibiotics are urgently needed. Many marine bacteria have been shown to produce secondary metabolites that display antibacterial properties. The first antibiotic from a marine bacterium was identified and characterized in 1966. In addition, bacteria in biofilm on the surface of marine organisms have been documented to contain a higher proportion of antibiotic producing bacteria than some other marine environments (Burgess, et al., 1999). Marine epibiotic bacteria, associated with nutrient-rich algal surfaces have also been shown to produce antibacterial secondary metabolites which inhibit the settlement of potential competitors. Recently a lot new novel antibiotics such as Phenazine, thiomarinol, phenazine-1-carboxylic acid, 1-hydroxyphenazine 2-n-heptylquinol-4-one, 2-n-nonylquinol-4-one pyolipic, loloatins, agrochelin, sesbanimides, pelagiomicins, indomycione and indomycione have been identified from various marine epibiotic bacterial organisms. In particular, some species of the genus Pseudomonas produce both antibiotics and several other bioactive substances. For example, Pseudoalteromonas rubra and Pseudoalteromonas aurantia have been reported to be antibiotic producing bacteria. The phenomenon of higher organisms utilizing their associated microflora for the production of beneficial secondary metabolites is common in the marine environment (Yotsu, et al., 1987). A study of bacteria isolated from marine algae surfaces indicated that the incidence of antibiotic producing strains from this habitat was 20% whereas that from sea water was only a few percent. In addition, some bacteria that previously did not produce any active compounds have been found to be producing such metabolites when they are exposed to other bacterial species or extra cellular chemical from other bacteria. Bacteria may also produce antimicrobial compounds when they sense the presence of competing organisms. However, few attempts have been made to study such chemical communication between different bacterial species or how this might affect. The secretion of antimicrobial compounds (Mearns-Spragg, et al., 1998). Bacterial communication by the chemical signals for specific function is simply known as Quorum sensing. In which a bacterial population receives input from the environment and elicits an appropriate response (Hiroaki and Kristina. 2003). The term "quorum sensing" describes the ability of a microorganism to perceive and response to diffusible signal molecules. Bacterial cells sense their population density through a sophisticated cell to cell communication system and trigger expression of particular genes. Tne first system of density-dependent regulation was studied in detail with the luminescence of Photobacterium fischeri (formerly known as Vibrio fischeri) by Bassler et al., 1997. Eventually, they discovered that 3-oxo-N-(tetrahydro-2-oxo-3-furanyl) hexanamid or N-3-(oxohexanoyl) homoserine lactone (OHHL) was responsible the agent in the broth that induced luminescence. Followed by this many researchers have confirmed that in Gram negative bacteria acyl-homoserine lactone is responsible for the cell to cell communication system.

In this present work the totally 54 isolates were screened from 20 different seaweed species out of which 34 species were showed the signs of quorum sensing i.e. 17 producer and 17 inducer strains, but when these organisms where subjected to quorum sensing principle in mixed culture only 3 them have responded. So the present study reveals around 17% of bacterial species isolated from seaweeds and sediment were responded to quorum sensing. According to the results of bacteria isolated from marine algae surfaces indicated that the incidence of antibiotic producing strains from this habitat was 20% where as that from sea water was only a few percent. In the present study also reveals more or less the same ratio in Pseudomonas spp. was observed. Our results also reveals a results of Kell et al., 1995; Stead et al., 1996; they have said the culture supernatant of Pseudomonas sps known to contain AHLS which induces the production of phenazine antibiotics. In this investigation due to time constraint, It was not attempted to identify the active compound secreted by Pseudomonas through quorum sensing, which may leave the space for the further intensive research in future.

What does this mean? Let yourself get a little dirty and scruffy for your women. Fix her car with your shirt off or get hot and sweaty over a game of basketball. Either way the scent of sweat and the salty taste of your skin will drive her wild!

In this experiment among 17 Producer and Inducer strains only 3 of them have responded to the quorum sensing principle. (BrB+/BrB-), (GcC+/GcC) and (SA+/SA-)

Once you've developed a strong bond with one another, she'll be looking forward to showing you a sneak peak of her wild side. The club is just the right place for it! - Natalia, Moscow

Yotsu, T., Y.Meguro, A. Endo, M. Murate, H. Naold, and T. Yasumoto.1987. Production of tetrodotoxin and its derivatives by Pseudomonas sp. Toxicon 25: 225 - 228

a) Among these 17 producers strain 6 strains were isolated from Hypnea musiformis. 6 from Gracillaria edulis, 4 from Ulva lactuca & 1 from Sediment.

Rheinheimer, G.1992.Aquatic microbiology (Wiley, New York), 3rd Ed. Stead, P., B. A. M. Rudd, H. Bradshaw, D. Nobel, and M. J. Dawson. 1996. Induction of phenazine biosynthesis in cultures Pseudomonas aeruginosa by L-N-(3-oxohexanoyl) homoserine lactone. FEMS Microbiology Letters 140: 15-22.

Did you ever believe that there was so much to learn about pheromone pest control? Neither did we! Once we got to write this article, it seemed to be endless.



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The erotic pleasure that can be derived from swinging is immeasurable. Exploring the side of our sexual nature with our partner and another couple, engaging in group sex, threesomes or all kinds of sexual activities just goes to prove that we are indeed sexual beings and that some of us really feel comfortable with what we are. Swingers represent the kind of people that prefer to go a little further and penetrate into some of the most incredible and almost bewildering sexual fantasies. They find swingers groups with the help of swingers ads and share common thoughts planning meetings and intimate encounters. They just love to be satisfied and most of them have declared that their existing relationships have deeply improved after such experiences.

The Quest for Fulfillment The quest of each individual, in this modern age, is arise out of the ashes of ancient myth, to slough off today's culture of "trying"; to turn sharply away from the lies of an under-achieving culture; to find the rough, perilous way to your soul. For it is the satisfaction of your soul that you are missing. No amount of "trying to make it happen" is going to get you there. Only finding the heroic core buried in your soul, your reason for existence, your drive to be a true Hero, can set you on the path to fulfillment... your soul desperately seeks you to touch this sleeping Lover.

Then the quest changes, for the energy expended thereafter is to keep lips locked together, never allowing a sliver of light to come between them. This is the new, and greater quest. For, winning the heart of a sleeping Lover is one thing. Keeping the heart of an awakened Lover quite another. And if you are not prepared for this new consuming task before you begin, you will have scant time for reflection to do it once you have slipped endlessly into her soul. The Hero must prepare diligently. Or extinguish all hope of merging with the awakened, fully aware Lover.

Awaken The Lover Who awakens the soul of the Lover? Who has the magic potion to open her eyes wide and seek her consuming embrace? What first put the Lover to sleep? Where was the magic potion hidden? Who chose this Hero to apply it?

ABSTRACT Bacterial biofilms are sessile communities with high cell density that are ubiquitous in natural, medical, and engineering environments; they are fascinating since they are primitive tissues with an advanced chemical communications network. Currently, there is an explosive amount of biofilm research, most of it with the ultimate aims of biofilm prevention, control, or eradication.The present investigation was aimed to study the cell signaling principle among seaweed epibiotic bacterial organisms , totally 54 bacterial isolates were made out of 20 seaweed species. Among 54 bacterial isolates 17 of them producer, another 17 of them inducer strains rest of 20 isolates were normal that have not showing any signs of activity. The 17 producers and 17 inducer strains were subjected to the cross species induction by quorum sensing principle and found that only 3 of them have responded to the cross species induction in production of antibacterial compound against the respective inducer strains. All the 3 producers and inducer were identified by biochemical analysis and surprisingly that all the 3 producer strain belongs to Genus Pseudomonas and inducer strains were belongs to Genus Vibrio. The supernatant obtain from mixed culture of Pseudomonas & Vibrio shows the antibiotic activity against the various Vibrio species which were isolated from other sources. Such as Vibrio from primary film, Vibrio from sediment Vibrio from seaweeds epibiotics. The obtain results clearly shows the particular Pseudomonas from seaweed epibionts has the capability of producing potential antibiotic compound against wide range of Vibrio species through quorum sensing.

My boyfriend uses this technique to get me oh so horny! First, he gently lays me on the bed , tells me to relax, and close my eyes. He softly wraps the blindfold over my eyes; then begins to caress my skin with his fingertips. Since, my sense of sight is gone, the rest of my senses are heightened. His every touch feels exciting and erotic. He goes on to remove my clothes, one by one and kisses my bare skin as he moves along. I never know what he is going to do next or where he is going to touch. My favorite is when he when he dips his finger in sensitizing oil and strokes the outside of my vagina softly then firmly. This technique is just what it takes for me to beg him to give it to me! I recommend men using this technique as well. Once your fingers land on the right spot, your women will be throbbing with arousal; she'll beg you for sex! - Ariana, CO

The Hero she seeks must prepare himself for her awakening long before the opportunity to win her wafts into the air. It is not his fortune that she seeks. It is not his beauty - for her Hero, her champion, will always be beautiful to her, no matter his visage. It is not his courage to best all in his quest to find her that underlies her dormant love.

Now, all my girlfriends are jealous of my sex life! - Carolina, SC 5. Listen to Reggaeton I'm from Puerto Rico, the home of Reggaeton, a new genre of music also hitting the United States. Reggaeton is a mix of Latin reggae and rap. The lyrics and rhythm of reggaeton songs are extremely sexual and erotic. The artists in these songs describe candidly what they want to do to women. Lyrics such as, "Te suelto el pelo, te quito la camisa, tu pantal??n y despu??s, te como completa," describe a scene where a man unties his woman's hair, takes off her shirt, her pants, and performs oral sex on her. Men should play reggaeton during sex to unleash wild fantasies in a woman's mind.

CONCLUSION: The present investigation was aimed to study the quorum sensing principle among seaweed epibiotic bacterial organisms. In the past few decades there was no findings of new novel antibacterial class compounds were identified. But, the pathogenic microorganisms show much higher rate of resistant development even to the potential antibiotics. So, there is an urgent need to discover new novel antibiotic compounds. The marine inhabitants such as microorganisms, seaweeds, invertebrates, etc., act as an undepleted source of wide range of natural products among which the seaweeds act as a potential source of antibiotic compounds. Currently the cross species induction / quorum sensing attracts the total attention of researchers in finding new novel drugs against multidrug resistant pathogenic microorganisms. So, the present study aim to find out the capability of seaweed epibiotic bacterial organisms to produce novel drugs against the animal and plant pathogens

Holmstrom, C, and S. Kjelleberg. 1994. The effect of external biological factors on, settlement of marine invertebrates and new antifouling technologies. Biofouling. 8: 147-160.

You've tried everything and still can't get her in the mood? Not to worry. In my duty to provide men with the most accurate information on female sexuality, I decided to conduct a nation wide survey.

This is the secret world of the undisclosed Lover. This is the place the Lover has chosen to launch her crusade to catch eternal love from the clutches of the ordinary world. She has chosen to sleep in this world for there is none within it to entice her to stay. But with her sleep she knows her sleeping pheromones will arouse only those most worthy of its essence, most able to arouse her and consummate the reason for her long sleep.

Burgess, J. G., M. Elizabeth, M. Bregu, A. Meams-Sprugg, and K. G. Boyd. 1999 Microbial antagonism: a neglected avenue of natural products research. J. Biotechnology. 70. 27-32.

BACTERIAL IDENTIFICATION The organisms responded to the quorum sensing process alone were identified by the following biochemical analysis.

*Corresponding author e-mail: vijay10bas@yahoo.co.in INTRODUCTION We are living in a microbial planet. About 71 % of the surface of this planet is covered by sea water. A typical milliliter of seawater contains 103 fungal cells, 106 bacteria, and 107 viruses, including pathogens that cause widespread mortalities and microbes that initiate fouling of host surfaces (Rheinheimer, 1992). Thus, marine plants and animals are continually exposed to high concentrations of potentially harmful microbes. These microorganisms in nature exists as free living planktonic mode of life in sea water or it may exist as epibiotic organisms in various living and nonliving surfaces. Among living organisms, seaweeds and invertebrates act as suitable substrate for the establishment of epibiotic organisms Seaweeds are known to release a large amount of organic carbon into the surrounding environment providing a nutrient rich habitat for microorganisms like bacteria. Bacteria are generally considered to be independent unicellular organisms. One cell accomplishes all of the tasks of feeding, locomotion, 'reproduction, respiration and all other processes necessary to keep an organism alive. There are several classes of bacteria such as primary film forming bacteria, sediment bacteria, symbiotic bacteria, and epibiotic bacteria in various aquatic organisms. The marine surface environment is a site of intense composition for living space by a wide variety of organisms. Bacteria are generally recognized as primary colonizers of this habitat and are able to form biofilm on marine surface such as invertebrates and algae (Bryers, et al., 1982). Bacteria may also be abundant on the surfaces of some algae as an important epibiotic organism. In many cases, the bacterial population found to be specific, with changes occurring throughout the year or life span of the algal surface. This algal-bacterial relationship is symbiotic in most cases; the epibiotic bacteria in seaweed play a protective role by releasing secondary metabolites into the surrounding seawater that help preventing extensive fouling of the surface. Epibiotic bacteria are therefore attracting attention as a source of new natural products. Bacteria from the larvae of some crustaceans protect them from fungal infection by the production of simple antimicrobial compounds. Bacteria isolated from the surface of a tunicate prevented the settlement of barnacle and tunicate larvae exposed to the bacteria as biofilm in petridishes (Evelyn et al., 2001).


?? 2007 Boyd L. Jentzsch



The end. Hope this article on pheromone pest control provided you with substantial information about it.
Author Dave Thomson
 
 
 


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