7/23/2019

The Benefits of Wearing Perfume 

Perfume has been used throughout history for a variety of reasons. Perfume has proved itself to be a valuable commodity in both ancient and modern times, with the modern perfume industry turnover in its billions every year. Perfume is a luxury, rather than a necessity, and yet its popularity grows rather than wavers every year. The reason behind this is the enjoyment that the wearer gains, and this type of enjoyment stems from the recognised and unnoticed benefits that wearing perfume provides.
 




It is only through sheer determination that we were able to complete this composition on nexus pheromone cologne. Determination, and regular time table for writing helps in writing essays, reports and articles.


Selective Pheromones Males are attracted to female pheromones. While male pheromones have been given the name of androstenone, no such name could be found to the female pheromones. It is referred by the same name and marketed through the brand names. In the world dominated by the males, where men are trying to woo females, it might be difficult to find a female trying to woo just any male. Women have to be specific and thus should be trying to woo a particular male by using a female pheromone.

Colony morphology, Gram staining, Motility test, Oxidase test, Catalase test, Indole Production, Methyl red test, Voges Proskauer test, Citrate Utilization test, Triple sugar Iron test, Nitrate reduction test, Lactose fermentation, Urease test

Microdictyon tenunis Chlorophyceae Chondrococcus hornemonii Chlorophyceae Enteromorpha intestinalis Chlorophyceae Caulerpa cupressoides Chlorophyceae

All the 17 strains were named as PRODUCERS STAINS BrA+, BrB+, BrC+, BrD+, BrE+, BrF+ Hypnea musiformis GcA+, GcB+, GcC+, GcD+, GcE+, GcF+ Gracillaria edulis

Burgess, J. G., M. Elizabeth, M. Bregu, A. Meams-Sprugg, and K. G. Boyd. 1999 Microbial antagonism: a neglected avenue of natural products research. J. Biotechnology. 70. 27-32.

Armstrong, E., J. D. Mckenzie, and G.T. G. Worthy. 1999. Aquaculture of sponges on scallops for natural products research and antifouling. J. Biotechnol. 70: 163-174. 70. 21-

Hypnea valentiae Rhodophyceae Hypnea pannose Rhodophyceae Hypnea esperi Rhodophyceae Acanthophora spicifera Rhodophyceae ISOLATION OF EPIPHYTIC BACTERIA

All the cultures were incubated at 28??C for 5 days. After the incubation period the cultures were centrifuged at 10,000 rpm for 15mins. The supernatant was collected and subjected to antibacterial assay with respective inducer strain.

Boyd, K.G., A. Mearns -Soragg, G. Briedley, K. Hatzidimitriou, A. Rennie, M. Bregu, M. 0. Hubble, and J. G. Burgess. 1998. Antifouling potential of epiphytic marine bacteria from the surface of marine algae. Plouzane, France: 128 -136.

This article is under GNU FDL license and can be distributed without any previous authorization from the author. However the author's name and all the URLs (links) mentioned in the article and biography must be kept.

Human Pheromones The existence of human pheromones was found in 1986 and then onwards it has become an industry in itself. The manufacture of pheromones has become a multi million dollar business. The research and development in pheromones is also a booming business in USA.

Firstly, the effect of perfume on our brains is not necessarily recognised though the effects are certainly enjoyed. Each scent, whether natural or synthetic, emits molecules which enter the nasal passages and eventually reach the olfactory epithelium. This part of the nose has a vast amount of receptor neurons which are each covered in hairs called cilia. These cilia trap the scent and send a message to the brain which enables a person to recognise the perfume. The ability to smell immediately has a positive impact. This is important as some research has shown that people without a sense of smell have a less happy life than those who can smell; scents trigger emotions and affect our mood.

As a result of this finding, perfume has become as much a science as it has an accessory. Aromatherapy studies the effects of natural botanical perfume ingredients, usually in the form of oils in their purest state. Its looks at the effects upon mood and general well being, and applies the knowledge by improving the perfume in the environment and offering a variety of aromatherapy services aimed at improving people`s lives. Aromacology applies this same approach to any available scent, from flowers to sand, and the findings are currently assisting perfume manufacturers worldwide.

What effects must I anticipate with a pheromone cologne? A marked increase in eye contact, interest and smiles from the opposite sex Increased sex appeal More dates and lovemaking A restoration of your existing relationship More ardent lovemaking A soothing, effortless effect on people around you More instances of being approached by the opposite sex Improved self confidence Spontaneous respect and attention on you by others.

Male Female Attraction through Pheromones The only way the lower living beings like ants, butterflies, birds, dogs or even a tiger can communicate is through pheromones. The pheromones are the way they communicate as they cannot communicate through oral or written word. The communication starts when the particular species it at its very best to procreate and ends when the species is not in a position to attract the opposite sex. Apart from the natural desire to find a mating partner, the pheromones also serve the purpose of communicating fight or flight signal.

Meains - Spragg, A., M. Bregu, K. G. Boyd, and J. G. Burgess. 1998. Cross species induction and enhancement of antimicrobial activity produced by epibiotic bacteria from marine algae and invertebrates, after exposure to terrestrial bacteria. Letters in Applied Microbiology. 27: 142-146.

Cappuccino J. G., and N. Shermann. 1999. Microbiology, A Laboratory manual, Fourth edition, Addison Wesley, New York. Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998. The involvement of cell to cell signals in the development of a bacterial biofilm. Science. 280: 295-298.

TEST ORGANISMS: 1. Epiphytic Vibrio from seaweeds 2. Vibrio from primary film 3. Vibrio from Sediments 4. Pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella sp. and Proteus sp

b) Among these 17-inducer strain 6 strains were isolated from Hypnea musiformis, 6 from Gracillaria edulis, 4 from Ulva lactuca & 1 from sediment.

The ingredients used in perfume may improve your concentration and enhance your mood. There is a long list of scents that are said to trigger this stimulating effect. Perfumes that contain ingredients such as ginger, sandalwood and rose are claimed by aromatherapists and aromacologists alike to have a stimulating effect on brain neurons. The increased stimulation aided by the use of perfume assists in many everyday tasks, from more focused studying to extra stamina and energy, balanced weight loss to an improved immune system.

Table 2: The results of Quorum Sensing analysis of epibiotic bacterial isolates from seaweeds. Seaweed sample Producer organism Inducer organism Cross-species producer with inducer Cross-species supernatant test with inducer Zone of clearance (mm)

Dictyota batryensis Phaeophyceae Sargassum sps Phaeophyceae Hypnea musciformis Rhodophyceae Acanthophora dendroides Rhodophyceae Jania rubens Rhodophyceae

In the present investigation, it was aimed to produce the antibiotics from the seaweed epibionts through quorum sensing principle. The bacterial isolates of seaweed epibionts were identified as species of Pseudomonas and Vibrio from seaweeds Hypnea musiformis and Gracillaria edulis. In this study the Pseudomonas acts as a producer strain and Vibrio as inducer strain. The recent finding says that the seaweed epibionts having potential to control the metabolic activity of competitor organisms. Allison et al., 1998 reported that many bacterial strains up on attaching to a surface produce exopolysaccharides or exopolypeptides. In addition, it has been postulated that exopolysaccharides could mediate the attachment of the bacteria to the surface and induce metabolic changes.

Bainton, N. J., P. Stead, and S.R. Chhabra. 1992. N-(3-oxohexanoyl)-L-homoserine Lactone regulated carbapenem antiobiotic production in Erwinia carotovora. Biochem. J. 288: 997-1004.

If we possess our own pheromones, what is the reason we are in need of a pheromone cologne? Because of our cultural practices... What do we engage in first thing in the morning, every morning? Take a shower. Then we put on underarm roll-ons and perhaps lotions or aftershaves which further screen our natural body chemistry. Next, we tend to cover up from head to toe, thereby veiling ninety per cent of the very skin which releases pheromones into the air. A few researches have also revealed that via evolution, our natural pheromone production has gradually decreased, as civilizations have relied on social customs and norms when selecting a partner.

Patterson, G. L., and C. Ml. Bolis. 1997. Fungal cell - wall poly sacchraides elicit an antifungal secondary metabolite in the Cyanobacterium Scytonema ocellatum. J. Phycology. 33: 54 - 60.

What is the reason for pheromone products? This may be the thing that offers you the competitive edge and the self-assurance you need to reach your social and sexual objectives, whatever they may be. Pheromones are a simple way to boost your attractiveness, and then match it with the confidence you'll have from all of the choices and interest.

The benefits of perfume are not just highlighted by scientific study. Many psychologists state that humans begin to store information about scents when only a few months old, and that scents affect reactions to people and events more than any of the other senses at this young age. In fact, our taste in smells remains more or less the same whereas tastes influenced by other senses are extremely changeable. This means that we choose perfumes for a reason, either it triggers a feeling of nostalgia or makes us feel a certain way. The perfume chosen also has a social relevance; it tells people something about the wearer, and helps cement their identity. Attraction to another person is influenced by invisible scents known as pheromones, and perfume adds to this. Above all, perfume makes the wearer feel fresh, attractive and more confident; and confidence is a definite benefit.

Dekievit, T. R., and B. H. Iglewski. 2000. Bacterial quorum sensing in pathogenic relationships. Infect. Immun. 66: 4839-4849. Dimango, E., H. J. Zar, R. Bryan, and A. Prince. 1995. Diverse Pseudomonas aerugniosa gene products stimulate respiratory epithelial cells to produce interleukin-8. J. Clin-lnvestig. 96: 2204-2210.

It is only through sheer determination that we were able to complete this composition on nexus pheromone cologne. Determination, and regular time table for writing helps in writing essays, reports and articles.



Icebreaker Pheromone Cologne



Lemos, M. L, A. E. Toranzod, and L. J. Barja. 1986. Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds. Microbial Ecology. 11: 149-163.

The collected seaweed samples were thoroughly washed with sterile seawater to removes the loosely attached bacteria/particles. Seaweed fronds were scrubbed with sterile cotton swabs to obtain epiphytic bacteria. Epiphytic bacterial organism in the swab were inoculated in sterile peptone broth (50% sea water) and incubated at 28??C in an incubated shaker (220 rpm / min) for overnight. After the incubation period the enriched cultures were serially diluted up to 10-8 concentration and 200 microlitre of each diluted samples were transferred into the nutrient agar plate (50% sea water). The plates were incubated at 28??C for 5 days and the plates with crowded colonies were selected. In the crowded plates those colonies, which showed the sign of inhibition zone around its margin to the neighboring colony, were selected and considered as producer strain. The neighboring sensitive colonies were treated as inducer strain. Both producer and inducer strains were streaked repeatedly until to get pure culture. The pure culture were properly labeled and subjected to the quorum sensing analysis.

Liming, Y., K. G. Boyd, and D. R. Adams. 2003. Biofilm specific cross species induction of antimicrobial compounds in Bacilli. AppI.Envi.Microbiol, 69:3719-3727.

Wagner, V. E., D. Bushneil, and L. Passador. 2003. Micro array analysis of Pseudomonas aeruginosa quorum sensing regulons. J. Bacteriol, 185: 2080 - 2095.

Hypnea musiformis 1. BrA+ 2. BrB+ 3. BrC+ 4. BrD+ 5. BrE+ 6. BrF+ BrA- BrB- BrC- BrD- BrE- BrF- Br A+ /Br A-

How Pheromones Are Used for Attracting Males In order to make a complete woman out of you and to competing with other women around your choice male, it might be necessary to get the attention of the male. The dressing is one way of doing it, but when you are in company of other females who are all similarly dressed, you might find that pheromones work on a subconscious level without your man knowing it. He is attracted to you by the action of pheromones and only to you. Make sure that your other actions do not impede the work of pheromones

illaud, A. C, and B. Austin. 1990. Inhibition of the fish pathogen, Serratia liquefaciens by an antibiotic producing isolates of planococcus recovered from sea water. Journal of

The test organisms Vibrio species were isolated from seaweed as epiphyles, biofilm, sediment and puffer fish by using TCBS medium (Hi media) The pathogenic bacteria were collected from clinical laboratories.

ANTIBIOTIC ASSAYS Antibiotic activity was performed in duplicate using a standard paper disc diffusion method as well as well assay. In well assay 10mm in diameter wells were made in marine agar plates and the plates were swabbed with 16 hours old inducer strain. To these wells 200ul of cell free supernatant were added to each well. In paper disc assay the Watmann no.1 filter paper discs (6mm in diameter) were saturated with 200ul of cell free supernatant. The impregnant discs were Dlaced in the centre of the plates swabbed with test organisms. The plates were Incubated at 37??C overnight and observed for inhibition zone. The zone of inhibition was measured as the distance from the border of paper disc to the edge of the clear zone and expressed in mm.

Pheromones are chemical aroma signals that all mammals/individuals emit. Subconsciously sensed, Nexus pheromones provide data about your immune system, your fertility, your present state of sexual interest and many more. A number of these pheromones focus on the opposite sex, and naturally trigger attraction arousal and enthusiasm for sex. With the proper chemical-hormonal signals, she spontaneously, intuitively responds without even knowing what is luring her to you!

Burgess, J. G., E. M. Jordan, M. Bregu, and A. Mearns-spragg. 1999. Microbial antagonism: a neglected avenue of natural products research. J. Biotechnology. 70: 27-32.

CONCLUSION: The present investigation was aimed to study the quorum sensing principle among seaweed epibiotic bacterial organisms. In the past few decades there was no findings of new novel antibacterial class compounds were identified. But, the pathogenic microorganisms show much higher rate of resistant development even to the potential antibiotics. So, there is an urgent need to discover new novel antibiotic compounds. The marine inhabitants such as microorganisms, seaweeds, invertebrates, etc., act as an undepleted source of wide range of natural products among which the seaweeds act as a potential source of antibiotic compounds. Currently the cross species induction / quorum sensing attracts the total attention of researchers in finding new novel drugs against multidrug resistant pathogenic microorganisms. So, the present study aim to find out the capability of seaweed epibiotic bacterial organisms to produce novel drugs against the animal and plant pathogens

Seaweeds itself secretes secondary metabolites to prevent fouling and grazing. In addition to that epibiotic bacteria on macro algae can also produce antifouling compounds that work in concert with the seaweed derived compounds to protect the seaweed surface. Recent studies have highlighted important roles of epibiotic bacteria colonizing the surface of seaweeds and releasing antifouling compounds. For the past 50 years antibiotics have revolutionized medicine by providing cures for formerly life threatening diseases. However, strains of bacteria have recently emerged that are virtually unresponsive to antibiotics such multidrug resistance, arising mainly through antibiotic misuse, is now recognized as a global health problem. In this situation, it is clear that new classes of antibiotics are urgently needed. Many marine bacteria have been shown to produce secondary metabolites that display antibacterial properties. The first antibiotic from a marine bacterium was identified and characterized in 1966. In addition, bacteria in biofilm on the surface of marine organisms have been documented to contain a higher proportion of antibiotic producing bacteria than some other marine environments (Burgess, et al., 1999). Marine epibiotic bacteria, associated with nutrient-rich algal surfaces have also been shown to produce antibacterial secondary metabolites which inhibit the settlement of potential competitors. Recently a lot new novel antibiotics such as Phenazine, thiomarinol, phenazine-1-carboxylic acid, 1-hydroxyphenazine 2-n-heptylquinol-4-one, 2-n-nonylquinol-4-one pyolipic, loloatins, agrochelin, sesbanimides, pelagiomicins, indomycione and indomycione have been identified from various marine epibiotic bacterial organisms. In particular, some species of the genus Pseudomonas produce both antibiotics and several other bioactive substances. For example, Pseudoalteromonas rubra and Pseudoalteromonas aurantia have been reported to be antibiotic producing bacteria. The phenomenon of higher organisms utilizing their associated microflora for the production of beneficial secondary metabolites is common in the marine environment (Yotsu, et al., 1987). A study of bacteria isolated from marine algae surfaces indicated that the incidence of antibiotic producing strains from this habitat was 20% whereas that from sea water was only a few percent. In addition, some bacteria that previously did not produce any active compounds have been found to be producing such metabolites when they are exposed to other bacterial species or extra cellular chemical from other bacteria. Bacteria may also produce antimicrobial compounds when they sense the presence of competing organisms. However, few attempts have been made to study such chemical communication between different bacterial species or how this might affect. The secretion of antimicrobial compounds (Mearns-Spragg, et al., 1998). Bacterial communication by the chemical signals for specific function is simply known as Quorum sensing. In which a bacterial population receives input from the environment and elicits an appropriate response (Hiroaki and Kristina. 2003). The term "quorum sensing" describes the ability of a microorganism to perceive and response to diffusible signal molecules. Bacterial cells sense their population density through a sophisticated cell to cell communication system and trigger expression of particular genes. Tne first system of density-dependent regulation was studied in detail with the luminescence of Photobacterium fischeri (formerly known as Vibrio fischeri) by Bassler et al., 1997. Eventually, they discovered that 3-oxo-N-(tetrahydro-2-oxo-3-furanyl) hexanamid or N-3-(oxohexanoyl) homoserine lactone (OHHL) was responsible the agent in the broth that induced luminescence. Followed by this many researchers have confirmed that in Gram negative bacteria acyl-homoserine lactone is responsible for the cell to cell communication system.

Why is it that I haven't learned of about this fascinating new sense organ and human pheromones before? Because it's a recent discovery! Although you may not have known about pheromones and the sixth sense until this time, you will: They're swiftly becoming common words.

GcA-, GcB-, GcC-, GcD- GcE-, GcF- Gracillaria edulis U1-, U2-, U3-, U4- Ulva lactuca SA- Sediment

Rheinheimer, G.1992.Aquatic microbiology (Wiley, New York), 3rd Ed. Stead, P., B. A. M. Rudd, H. Bradshaw, D. Nobel, and M. J. Dawson. 1996. Induction of phenazine biosynthesis in cultures Pseudomonas aeruginosa by L-N-(3-oxohexanoyl) homoserine lactone. FEMS Microbiology Letters 140: 15-22.


Wearing female pheromones is not an end all activity. Without the attendant personality and natural attraction it might not work well, but when you are competing with equals, wearing a pheromone will definitely give you the edge.



This is our humble presentation on nexus pheromone cologne. Your reading it will add the necessary weightage to the presentation.
Author Dave Thomson
 
 
 


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